Protein and nucleic acid interactions

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facet applications:Protein and nucleic acid interactions

facet content type:Protocol

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RNA Immunoprecipitation qPCR (RIP- qPCR) Protocol

RNA immunoprecipitation (RIP) can identify specific RNA molecules of many types associated with specific nuclear or cytoplasmic binding proteins.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Protocol for Anti Ago-RNA Immunoprecipitation from Mammalian Cells Using the RIP Kit

Procedure and protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit

Chromatin Isolation by RNA Purification (ChIRP) Protocol

Chromatin Isolation by RNA purification (ChIRP) protocol for isolating RNA-bound genome regions.

Creating PLA® Probes

Duolink® kits use in situ PLA® technology for accurate quantification of individual proteins, interactions, and modifications in cells and tissue.

Using Duolink® in Protein Interaction Studies

The video follows the simple and straightforward procedure that allows you to detect, quantify and obtain cell localization of protein interactions and their modifications in a single experiment.

Duolink® PLA Brightfield Protocol

Duolink® PLA reagents enable brightfield detection and quantification of proteins and interactions in tissue samples.

Counterstaining after the Duolink In Situ Protocol

We recommend applying the counterstaining protocol after the completion of the Amplification step in section 7.3, step 5 of the Duolink In Situ Fluorescence User Manual.

Duolink® PLA Multicolor Detection Protocol

Duolink® PLA Probemaker Guide

Guide to create custom PLA probes for immunofluorescent or brightfield detection.

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