Protein labeling and modification

应用筛选条件

facet applications:Protein labeling and modification

facet content type:Protocol

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酶法去糖基化

酶法去糖基化策略：从多糖中连续水解单个单糖可用于阐明多糖组分的结构和功能。

聚糖的质谱

该聚糖质谱分析方案，被称之为干液滴法，基于原始的MALDI实验并依然是MS领域中最常用的方法。

用于核酸去磷酸化的小牛肠（CIP）碱性磷酸酶

CIP可用于脱去DNA、RNA及核糖和脱氧核糖核苷三磷酸上的5’-磷酸基团。如何进行DNA去磷酸化的详细实验方案。

方案指导：使用PKH亲脂性膜染料标记外泌体

PKH染料已成功用于体外和体内跟踪实验中标记外泌体和细胞外囊泡。下述外泌体标记方案详细介绍了在微囊泡追踪实验中使用PKH染料实现可靠的外泌体荧光标记的步骤。

Amicon Ultra超滤管纯化抗体标记反应物

有了Amicon® Ultra标准式离心超滤管，不再需要对样品进行稀释，只要经过几轮洗滤操作，就能有效去除抗体标记反应中的未结合标记物。

Clean-up of Antibody Labeling Reactions Using Amicon Ultra Filters

Using a standard Amicon® Ultra centrifugal filter, a few rounds of diafiltration can efficiently remove unincorporated label from an antibody labeling reaction without diluting the sample.

Protocol Guide: Exosome Labeling Using PKH Lipophilic Membrane Dyes

PKH dyes have been successfully used to label exosomes and extracellular vesicles for in vitro and in vivo tracking experiments. The exosome labeling protocol below details steps for reliable fluorescent labeling of exosomes using PKH dyes for microvesicle tracking experiments.

Purification or Removal of Biotin and Biotinylated Biomolecules with Magnetic Beads

This page shows how to Purify or remove biotin and biotinylated biomolecules with magnetic beads.

Coupling Protocol for Primary Amine of a Ligand

This page shows coupling through the primary amine of a ligand with a NHS-activated Sepharose High Performance, NHS-activated Sepharose 4 Fast Flow and CNBr-activated Sepharose from Cytiva.

Purification or Removal of Biotin and Biotinylated Substances

This page shows how to purify or remove biotin and biotinylated substances with Streptavidin Sepharose High Performance from Cytiva.

N-Glycosidase F Protocol & Troubleshooting

Neuraminidase (Sialidase) Protocol

Neuraminidase can be used to cleave sialic acids from proteins. In this protocol, the enzyme from Vibrio cholerae is used on fixed cells.

Coupling to Microparticles

Biochemicals Coupling to Microparticles. Besides Proteins, DNA, lectins and many other species have been coupled to microparticles.

Conjugating Alkaline Phosphatase to Antibodies and Other Proteins

Protocol for the one-step, glutaraldehyde coupling method to conjugation alkaline phosphatase to antibodies and other proteins.

Tag Removal by Enzymatic Cleavage

This protocol shows how to remove histidine tags by enzymatic cleavage using Cytiva products.

Peptide Quick Tips

Peptide Quick Tips. Helpful guidelines for proper storage and resuspension of your peptide for optimal performance in your assay.

Mass Spectrometry of Glycans

The development of modern mass spectrometry (MS) equipment with high resolution and mass accuracy has led to its use in analyzing glycans for both profiling and structural studies.

Alkaline Phosphatase Protocol

Alkaline phosphatase (AP) is a non-specific phosphomonoester hydrolase that catalyzes the hydrolysis of a wide variety of organic monophosphates.

Enzymatic Deglycosylation

Enzymatic Deglycosylation Strategies: Sequential hydrolysis of individual monosaccharides from glycans can be useful for the elucidation of the structure and function of the glycan component.

Calf Intestinal (CIP) Alkaline Phosphatase for Nucleic Acid Dephosphorylation

CIP is used to remove 5’-phosphate groups from DNA, RNA and both ribo and deoxy-ribonucleoside triphosphates. Detailed protocol on how to dephosphorylate DNA.

Removal of GST Tag by Enzymatic Cleavage

This page how to remove GST tags by enzymatic cleavage using Cytiva products.