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Protein labeling and modification

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facet applications:Protein labeling and modification
facet content type:Technical Article
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固相合成
多肽是采用固相FMOC或BOC化学法在PEG-聚苯乙烯载体树脂上合成的。合成完成后,先去除侧链保护基团,同时从树脂上切割多肽。随后,将经切割和去保护的多肽材料沉淀、清洗并溶解在含H2O/ACN/HOAC的缓冲液中,然后进行冻干处理。
Ni-NTA-Atto共轭物
Ni-NTA-Atto共轭物为 His 标记的融合蛋白提供特异性和高灵敏度的检测。
肽偶联剂选择指南
Novabiochem®可提供大量的偶联剂用于原位活化。原位活化试剂易于使用 - 即使是利用空间位阻氨基酸,并且它们的使用通常没有副反应。
用于抗体表征的酶
抗体现今是治疗药物中成长最快的类别
聚糖标记
蛋白质的结构修饰对于活细胞是必不可少的。发生调节异常时,它们往往是会引发疾病。聚糖是生物系统中许多结构变异的原因,它们在细胞表面的表现通常被称为“糖”。
用于固相肽合成的ChemMatrix®树脂
ChemMatrix®是PCAS BioMatrix专有的一种基于100%PEG(聚乙二醇)的树脂。
用于不对称合成的手性胺
手性胺已广泛用于不对称合成,例如用作对映选择性去质子化反应中的手性碱,或用于拆分消旋的酸。
自组装单层:烷硫醇的优点
我们提供了一篇关于自组装单层的文章:烷硫醇的优点。
O-连接糖蛋白回顾
O-连接糖蛋白通常是分子量>200kDa的大蛋白。糖基化通常发生在高密度簇上,并可占总量的50-80%。
N-连接聚糖概述
N-连接聚糖、修饰和降解
肽修饰:N-末端、内部、和C-末端
肽修饰:N-末端、内部、和C-末端
肽聚糖的结构、生物合成和功能
肽聚糖(PGN)的基本结构含有N-乙酰葡糖胺(GlcNAc)与N-乙酰胞壁酸交替单元的碳水化合物主链,其中N-乙酰胞壁酸残基与肽交联。
金纳米颗粒:性质和应用
金(Au)纳米颗粒具有可调的光学和电子性质,适用于许多应用,包括光伏、传感器、药物递送和催化。
Atto Dyes and Tracy Dyes for Fluorescent Protein Labeling
We offer protein labeling kits based on two types of fluorescent dyes, the Atto dyes and the Tracy dyes. Both series of kits provide an easy and reliable way to fluorescently label purified proteins, enzymes, and antibodies.
Ni-NTA-Atto Conjugates
Ni-NTA-Atto conjugates provide specific and highly sensitive detection of His-tagged fusion proteins.
ChemMatrix® Resin for solid phase peptide synthesis
ChemMatrix® is a proprietary, 100% PEG (polyethylene glycol) based resin from PCAS BioMatrix.
Solid Phase Synthesis
Peptides are manufactured using solid phase FMOC or BOC chemistry methodologies on a PEG-Polystyrene support resin. Upon synthesis completion, side chain protecting groups are removed and the peptides are simultaneously cleaved from the resin. The cleaved and deprotected
Chymotrypsin
Analytical Enzyme Chymotrypsin: Chymotrypsin is produced in the acinar cells of the pancreas as the inactive precursor, chymotrypsinogen.
Self-Assembled Monolayers: Advantages of Pure Alkanethiols
We presents an article regarding Self-Assembled Monolayers: Advantages of Pure Alkanethiols.
Utilizing ISOGRO® Supplementation of M9 Minimal Media to Enhance Recombinant Protein Expression
Utilizing ISOGRO® Supplementation of M9 Minimal Media to Enhance Recombinant Protein Expression.
Signal Peptide Optimization: Effect On Recombinant Monoclonal IgG Productivity, Product Quality And Antigen-Binding Affinity
A signal peptide is a 5-30 amino acid (aa) peptide present at the N-terminus of secretory proteins.
Enzymes for Antibody Characterization
Antibodies now make up the fastest growing category of therapeutic drugs
Post Translational Modification
Post-translational modifications such as glycosylation, phosphorylation, and sulfation, to name a few, serve many functions. As a result, the analysis of proteins and their post-translational modifications is particularly important for the study of diseases where multiple genes are known to
Chiral Amines in Asymmetric Synthesis
Chiral amines have found widespread application in asymmetric synthesis serving, for instance, as chiral bases in enantioselective deprotonation reactions or being valuable substances for resolving racemic mixtures of acids.
Peptide Modifications: N-Terminal, Internal, and C-Terminal
Peptide Modifications: N-Terminal, Internal, and C-Terminal
Cleavage and Purification of GST-Tagged Protein Bound to GSTrap
This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from Cytiva.
Unnatural Amino Acids for Peptide Synthesis
Unnatural amino acids, the non-proteinogenic amino acids that either occur naturally or are chemically synthesized, are becoming more and more important as tools for modern drug discovery research.
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