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Protein lysis and extraction
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facet applications:Protein lysis and extraction
facet content type:Protocol
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TRI Reagent® 实验方案
TRI试剂是一种单步RNA分离试剂，可同时分离DNA、RNA和蛋白质。本实验方案概述了样品制备方法，以及各种样品提取方法和故障排除方案。
WB检测样本前处理：细胞裂解和蛋白提取
Western blotting前用于培养组织和细胞的细胞裂解和有效蛋白提取的样品制备实验方案。
TRI Reagent® Protocol
TRI Reagent is a single-step RNA isolation reagent that allows simultaneous isolation of DNA, RNA and protein. This protocol outlines sample prep as well as extraction of the various samples and troubleshooting.
Nuclear Protein Extraction Without the Use of Detergent
Detergents can interfere with the labeling efficiency of the extracted proteins. Therefore, a procedure that does not include the use of detergents should be used for the preparation of nuclear proteins.
Protease Inhibition Using Protease Inhibitor Mix
Protease Inhibitor Mix from Cytiva inhibits serine, cysteine, metalloproteases, and calpain proteases and is suitable for animal and plant tissues, yeast, and bacteria.
Liberase™ DL Research Grade Protocol & Troubleshooting
Your current tissue dissociation protocol, and the extent to which you have been successful with traditional collagenase are good guides for selecting the appropriate Liberase Enzyme Blend.
Liberase™ TL Research Grade Protocol & Troubleshooting
Liberase™ TL Research Grade Protocol & Troubleshooting
Lysosome Isolation Kit
Lysosomes are organelles ubiquitously distributed in most pro- and eukaryotic cells. The Lysosome Isolation Kit provides a method for isolating lysosomes from animal tissues and from cultured cells by differential centrifugation followed by density gradient centrifugation and/or calcium precipitation.
Cell Disruption and Membrane Preparation
This page shows how to perform a cell disruption und prepare membranes with products from Cytiva.
Handling Inclusion Bodies in Recombinant Protein Expression
How to isolate proteins from inclusion bodies using Cytiva products.
cOmplete™, Mini, EDTA-free Protocol
Dissolve one tablet in 10 ml aqueous buffer or water. If high proteolytic activity is present, dissolve one tablet in 7 ml buffer.
Cell Lysis and Protein Extraction for Western Blotting
Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.
Liberase™ DH Research Grade Protocol & Troubleshooting
Your current tissue dissociation protocol, and the extent to which you have been successful with traditional collagenase are good guides for selecting the appropriate Liberase Enzyme Blend.
Liberase™ TH Research Grade Protocol & Troubleshooting
Your current tissue dissociation protocol, and the extent to which you have been successful with traditional collagenase are good guides for selecting the appropriate Liberase Enzyme Blend. Select the Liberase Enzyme Blend most similar to your tissue dissociation application, or
Processing Small Tissue or Cell Samples using Sample Grinding Kit
Sample Grinding Kit from Cytiva is designed to disrupt cell or tissue samples for protein extraction.