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Protein mass spectrometry
应用筛选条件
facet applications:Protein mass spectrometry
facet content type:Protocol
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聚糖的质谱
该聚糖质谱分析方案，被称之为干液滴法，基于原始的MALDI实验并依然是MS领域中最常用的方法。
阿达木单抗（adalimumab）肽图谱 LC-MS/MS 分析工作流程
基于优化的 LC-MS/MS 工作流程，通过过滤辅助样品制备 (FASP) 对阿达木单抗 (Humira) 绘制低伪影胰蛋白酶酶解和肽图谱。
Amicon® Ultra超滤离心管在MALDI质谱分析中纯化血清多肽
生物标志物在药物发现和开发中起关键作用，
Antibody Drug Conjugate Mimic Enables LC-MS Method Development Without Risk
Here we show how LC and MS methods may be optimized using a non-toxic surrogate of the ADC, an “ADC-mimic”, that behaves very similarly to the Cys-linked ADC Adcetris (Seattle Genetics).
Standard Methods 6040D: GC Analysis of Geosmin and 2-MIB on SLB®-5ms after SPME using 50/30 µm DVB/Carboxen/PDMS Fiber
Separation of 2-Isopropyl-3-methoxypyrazine 20 ppt; 2-Isobutyl-3-methoxypyrazine; 2-Methylisoborneol; (±)-Geosmin 10 ppt
LC-UV-MS Method Development for Antibody Drug Conjugates Using a Non-Toxic ADC-Mimic
SigmaMab Antibody Drug Conjugate Mimic, is a non-toxic drug mimic utilized as a standard for mass spectrometry and high performance liquid chromatography.
Peptide Mapping LC-MS/MS Analysis Workflow for Adalimumab
An optimized LC-MS/MS based workflow for low artifact tryptic digestion and peptide mapping of monoclonal antibody, adalimumab (Humira) using filter assisted sample preparation (FASP).
Protocol for Purification, Optional Reduction, and SEC-MS Analysis of a Monoclonal Antibody
A complete workflow for the intact and middle-up mass analysis of reduced and non-reduced monoclonal antibodies based on SEC-MS with sample preparation by protein-A affinity clean-up.
Purification of Serum Peptides for MALDI Mass Spectrometry with Amicon® Ultra Centrifugal Filters
Biomarkers play an essential role in the drug discovery and development process.
Workflow and Analysis of Stressed and Unstressed Antibody Samples By Size Exclusion Chromatography
A complete SEC-UV workflow for quantifying monoclonal antibody fragments, monomers, and aggregates, including procedure for a system suitability test and heat stressing of antibodies.
Production of 15N-13C Dual-labelled Proteins
Dr. Alan Robertson and Dr. Ben Davis at Vernalis, Ltd., UK, a provider of drug discovery and development services, have refined a novel protocol for the duallabelling of proteins using EnPresso® B Defined Nitrogen-free.
Mass Spectrometry of Glycans
The development of modern mass spectrometry (MS) equipment with high resolution and mass accuracy has led to its use in analyzing glycans for both profiling and structural studies.
LC/MS/MS Analysis of Aflatoxins in Cannabis on Ascentis® Express 2.7µm Phenyl-Hexyl after Cleanup Using Supel™ Tox AflaZea SPE
solution, 2 μg/mL in acetonitrile, analytical standard
Workflow for High-Throughput Glycoprofiling of Rituximab
Step-by-step SEC-MS protocol for rapid glycoprofiling of a monoclonal antibody (mAb), consisting of antibody purification, a mAb reduction procedure, a mass spectrometer calibration, and a system suitability test.