Breaking the Boundaries of Biomolecule Separation with BIOshell™ UHPLC Columns

WEBINAR

Superficially porous particles (SPPs) have advantages over traditional fully porous particles (FPPs) when packed into columns for chromatographic separation of biomolecules. Due to the presence of a solid silica core, analytes have a smaller distance to travel within a particle, thus minimizing diffusion due to mass transfer effects. Combined with a large pore diameter and a thin, porous shell, these particles can yield highly efficient peaks even for large proteins such as monoclonal antibodies.

In this webinar, we will discuss:

In this webinar, you will learn about the advantages of using SPP-packed BIOshell™ columns for various large molecule separation challenges. Aspects of peptide mapping, intact analysis and middle-up analysis will be reviewed, and strategies for improving these assays will be provided. In addition, a unique 1.5 mm I.D. column geometry and its advantages in improving sensitivity and making methods more sustainable will be reviewed. Finally, strategies for characterizing oligonucleotides will be discussed in relation to how BIOshell™ can assist in analyzing this class of biomolecule.

Key Learning Objectives

• Discover the importance of SPP particle architecture (pore size and shell thickness) and how it contributes to high-efficiency separations of biomolecules
• Explore how column chemistry can play a role in improving peptide mapping accuracy
• Learn how extra column dispersion can affect chromatographic performance and discover strategies to mitigate it