A0521
α-Amylase from human saliva
Type IX-A, lyophilized powder, 1,000-3,000 units/mg protein
Synonym(s):
1,4-α-D-Glucan-glucanohydrolase
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About This Item
CAS Number:
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-565-6
NACRES:
NA.54
MDL number:
Specific activity:
1,000-3,000 units/mg protein
type
Type IX-A
Quality Level
form
lyophilized powder
specific activity
1,000-3,000 units/mg protein
composition
Protein, 5.0-15.0% biuret
greener alternative product characteristics
Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
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UniProt accession no.
greener alternative category
storage temp.
−20°C
Gene Information
human ... AMY1A(276)
General description
α-amylase is the main secretory protein constituent of saliva. It contains 496 amino acids and has two isoforms, glycosylated and non-glycosylated. α-amylase gene is located on human chromosome 1p21.1. α-amylase is mainly produced in pancreas and saliva.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles.
Application
α-amylase from human saliva has been used to test α-amylase inhibitors using HPLC (high performance liquid chromatography). It also has been used to study the digestibility of human milk oligosaccharides (HMO).
Biochem/physiol Actions
α-amylase catalyses the hydrolysis of α-1,4 glycosidic linkage in oligosaccharides. It plays a crucial role in the initial digestion of starch, glycogen and polysaccharides. Elevated levels of amylase in serum is linked to acute pancreatitis.
Physical form
Lyophilized powder containing (NH4)2SO4 and sodium citrate
Preparation Note
Chromatographically purified
Other Notes
One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 6.9 at 20 °C.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
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Protocols
Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.
可根据此操作规程测定α-淀粉酶的活性。该α-淀粉酶酶促测定将会指导您完成整个过程及必要的计算。
M Alberta Araújo et al.
Biomaterials, 25(13), 2687-2693 (2004-01-31)
Apart from favourable physico-chemical and mechanical properties, the most important requirement for a biodegradable polymer to be used in medical applications is its biocompatibility and the non-cytotoxicity of its degradation products. Their combined effect should assure the safe material degradation
HPLC method for measurement of human salivary alpha-amylase inhibition by aqueous plant extracts
Takacs I, et al.
PLoS ONE, 68(2), 127-136 (2017)
M J Gnoth et al.
The Journal of nutrition, 130(12), 3014-3020 (2000-12-09)
In examining the functional aspects of human milk oligosaccharides (HMO), it is not known whether they are digested during the passage through the infant's gastrointestinal tract. HMO were prepared from individual milk samples (n = 6) and separated into neutral
Global Trade Item Number
| SKU | GTIN |
|---|---|
| A0521-500UN | 04061832841298 |
| A0521-100UN | 04061833334058 |
| A0521-2.5KU | 04061833334065 |