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A8582

Sigma-Aldrich

Antifoam SE-15

aqueous emulsion for bacterial and mammalian systems

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Synonym(s):
silicone-based antifoam
NACRES:
NA.85

Quality Level

200

form

emulsion (aqueous)

concentration

10% (active silicon)

technique(s)

cell culture | hybridoma: suitable

solubility

H2O: soluble (effective hot or cold.)

density

1.0 g/mL at 25 °C

application(s)

microbiology

storage temp.

room temp

suitability

(Mammalian (suspension))
bacteria (fermentation)

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General description

Antifoam SE-15 is a silicone-based product used to prevent or minimize foaming in both microbial fermentation systems and mammalian suspension cultures. Control of foaming minimizes damage to the cells, resulting in increased protein/antibody production.

Application

Antifoam SE-15 has been used:

  • in the preparation of ramified rolling circle amplification (RAM) amplification buffer.
  • to find its effects on cell viability, virus infectivity and PCR (polymerase chain reaction) performance.
  • as a constituent of phosphate-buffered saline (PBS) for the determination of total antioxidant status (TAOS) by 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay.
  • as a component of autoinduction expression culture.
  • as a component of cyclic adenosine monophosphate (cAMP) detection buffer.

Features and Benefits

  • Controls foaming in microbial culture flasks or fermentations
  • Non-toxic to the culture medium
  • Can be pumped to fermenter on an as-needed basis

Components

Antifoam SE-15 is an aqueous emulsion containing 10% active silicon and non-ionic emulsifiers .

Preparation Note

Antifoam SE-15 can be pre-diluted with 3-10 parts of cool water to aid in dispersion. Pre-diluted
suspensions should be used immediately.

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Elisa Binda et al.
BMC biotechnology, 13, 24-24 (2013-03-19)
VanYn, encoded by the dbv7 gene (also known as vanYn) of the biosynthetic cluster devoted to A40926 production, is a novel protein involved in the mechanism of self-resistance in Nonomuraea sp. ATCC 39727. This filamentous actinomycete is an uncommon microorganism
James H Smith et al.
PloS one, 8(5), e65053-e65053 (2013-06-01)
Isothermal amplification methods provide alternatives to PCR that may be preferable for some nucleic acid target detection tasks. Among current isothermal target detection methods, ramified rolling circle amplification (RAM) of single-stranded DNA circles that are formed by ligation of linear
Detection of nucleic acid targets using ramified rolling circle DNA amplification: a single nucleotide polymorphism assay model
Smith J H and Beals T P
PLoS ONE, 8(5), e65053-e65053 (2013)
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