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BCIP®/NBT solution, premixed

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NACRES:
NA.32

form

liquid

Quality Level

100

Related Categories

Application

BCIP/NBT solution, premixed has been used:
  • as a substrate to develop dot-blots
  • as a substrate to reveal alkaline phosphatase in the immunohistochemical analysis
  • to detect specific immune complexes in western blot analysis

Other Notes

0.48 mM NBT, 0.56 mM BCIP, 10 mM Tris and 59.3 mM MgCl2, pH approx. 9.2

Substrates

Substrate for visualizing alkaline phosphatase conjugates in Western blotting.

Legal Information

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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The major human structural IgE epitope of the Brazil nut allergen Ber e 1: a chimaeric and protein microarray approach
Alcocer M J C, et al.
Journal of Molecular Biology, 343(3), 759-769 (2004)
Sandra Fernandes Arruda et al.
Nutrients, 12(5) (2020-05-28)
The Brazilian savanna fruit, tucum-do-cerrado (Bactrissetosa Mart.) reduces hepatic hepcidin levels. Therefore, we investigated the effect of tucum-do-cerrado on the TfR/HFE and/or BMP/HJV/SMAD and JAK/STAT pathways, in normal and excess iron conditions. Rats were treated with: control diet (CT); control
Neuroprotective effects of recombinant human granulocyte colony-stimulating factor (G-CSF) in neurodegeneration after optic nerve crush in rats
Tsai R K, et al.
Experimental Eye Research, 87(3), 242-250 (2008)
Anti-apoptotic effects of human granulocyte colony-stimulating factor (G-CSF) on retinal ganglion cells after optic nerve crush are PI3K/AKT-dependent
Tsai R K, et al.
Experimental Eye Research, 90(5), 537-545 (2010)
Willie Lin et al.
Oncotarget, 9(2), 1992-2001 (2018-02-09)
CD34 is a transmembrane phosphoglycoprotein used to selectively enrich bone marrow in hematopoietic stem cells for transplantation. Treating rats with CD34
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Articles

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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