D7052
Dipeptidyl Peptidase IV from porcine kidney
lyophilized powder, ≥10 units/mg protein (Bradford)
Synonym(s):
Dipeptidyl aminopeptidase IV
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About This Item
form
lyophilized powder
specific activity
≥10 units/mg protein (Bradford)
mol wt
124 kDa
packaging
vial of ≥0.75 unit
solubility
0.1 M Tris-HCl, pH 8.0: soluble 1 vial/mL, clear, colorless
UniProt accession no.
storage temp.
−20°C
Gene Information
pig ... DPPIV(397492)
Specificity
DPPIV has a post-proline dipeptidyl aminopeptidase activity that hydrolyzes N-terminal dipeptides from the unsubstituted N-terminus of peptides with the sequence of X-Pro-Z and X-Ala-Z. Where X is a nonspecific residue at the N terminus and Z cannot be proline or hydroxyproline.
Application
Dipeptidyl peptidase IV from porcine kidney has been used in a study to investigate engraftment of donor cells following hepatocyte transplantation. Dipeptidyl peptidase IV from porcine kidney has also been used in a study to investigate the metabolism of glucagon-like peptide-2.
The enzyme from sigma has been used in the determination of DPP-IV inhibitory activity of peptides in porcine skin gelatin hydrolysates.[1]
Biochem/physiol Actions
Native DPPIV is a ubiquitous type II transmembrane glycoprotein and a serine protease of the S9 prolyl-oligopeptidase family. In vivo, it is synthesized with a signal peptide, which functions as the membrane anchoring domain. There is an 88% sequence homology between the human and porcine kidney enzymes. Both exist as homodimers with a subunit molecular weight of ~30 kDa. The high mannose 100 kDa DPPIV precursor is processed in the Golgi to yield a 124 kDa heavily N-and O-linked mature glycoprotein. It is then sorted to the apical membrane through the concerted action of both N- and O-linked glycans and its association with lipid microdomains. The porcine enzyme contains 18.3% carbohydrates, which the glycan composition is 0.9% fucose, 3.4% mannose, 5.1% galactose, 8.2% glucosamine, and 0.7% sialic acid. DPPIV is highly expressed on endothelial cells, epithelial cells, and lymphocytes. It is also present in plasma in its soluble form.
Unit Definition
One unit will produce 1.0 μmole of p-nitroaniline from Gly-L-Pro p-nitroanilide per min in 100 mM Tris-HCl at pH 8.0 at 37 °C.
Physical form
Lyophilized powder containing Tris buffer salts
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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