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General description
Application
- protein deglycosylation
- deglycosylation of tissue factor pathway inhibitor (TFPI) α and β
- deglycosylation of native exo-β-(1,3)-glucanase EXG1
Features and Benefits
- two or more mg of glycoprotein is deglycosylated
- single reaction at neutral pH
- native & denaturing procedures
- no degradation of protein
- O-linked sugars containing polysialic acid are eliminated
- control glycoprotein provided
Kit Components Only
- PNGase F 1 mL/vial
- O-Glycosidase 20 μL
- α-2(3,6,8,9)-Neuraminidase 20 μL
- Fetuin Control .5 mg
- 5× Reaction Buffer .2 mL
- Denaturation Solution .1 mL
- Triton™ X-100 .1 mL
- β-1→4-Galactosidase, positionally specific 20 μL
- β-N-Acetylglucosaminidase 20 μL
related product
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
Certificates of Analysis (COA)
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Aldrich Products
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Articles
Information about Glycoprotein deglycosylation. The diversity of oligosaccharide structures, both O-linked and N-linked, often results in heterogeneity in the mass and charge of glycoproteins.
Deglycosylation Kits
See how pretreatment with a mucin-selective protease, Mucinase StcE, for tryptic-digest sample preparation of mucins may increase the number of glycopeptides and glycoforms identified from your samples.
Post-translational modifications such as glycosylation, phosphorylation, and sulfation, to name a few, serve many functions. As a result, the analysis of proteins and their post-translational modifications is particularly important for the study of diseases where multiple genes are known to be involved, such as heart disease, cancer and diabetes.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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