EDEGLY
Enzymatic Protein Deglycosylation Kit
Synonym(s):
Glycosylation Release Kit
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About This Item
General description
Application
- protein deglycosylation
- deglycosylation of tissue factor pathway inhibitor (TFPI) α and β
- deglycosylation of native exo-β-(1,3)-glucanase EXG1
Features and Benefits
- two or more mg of glycoprotein is deglycosylated
- single reaction at neutral pH
- native & denaturing procedures
- no degradation of protein
- O-linked sugars containing polysialic acid are eliminated
- control glycoprotein provided
Kit Components Only
- PNGase F 1 mL/vial
- O-Glycosidase 20 μL
- α-2(3,6,8,9)-Neuraminidase 20 μL
- Fetuin Control .5 mg
- 5× Reaction Buffer .2 mL
- Denaturation Solution .1 mL
- Triton™ X-100 .1 mL
signalword
Danger
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3
Regulatory Information
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Articles
Information about Glycoprotein deglycosylation. The diversity of oligosaccharide structures, both O-linked and N-linked, often results in heterogeneity in the mass and charge of glycoproteins.
Explore strategies for releasing N-linked glycans with PNGase F, PNGase A & native & sequential deglycosylation with endoglycosidases & exoglycosidases.
Protein modifications are crucial for disease study. Analysis methods are key.
Related Content
Global Trade Item Number
| SKU | GTIN |
|---|---|
| EDEGLY-1KT | 04061833609392 |