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Merck
CN

G7663

Sigma-Aldrich

Gelatin blocking buffer

for Western blotting, powder blend

Synonym(s):

blocking buffer

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About This Item

UNSPSC Code:
41105300
NACRES:
NA.25

Key Documents

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grade

Molecular Biology

Quality Level

100

form

powder blend

storage temp.

room temp

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General description

Gelatin Blocking Buffer is a standard reagent in Western blotting procedures. It is used to block non-specific binding on the membrane, when using nylon or nitrocellulose (but not PVDF).

Application

Suitable for use as a non-specific blocking agent for Western blots.

Preparation Note

Dissolve entire contents of the bottle in a final volume of 1L molecular biology grade water. The resulting solution will be faint yellow in color and does not require filtering or heating before use.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
SLBR8508V
SLBP6251V
SLBN9526V
SLBD9944V
SLBD9944

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Daisuke Tomida et al.
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A Fortin et al.
Biochemistry and cell biology = Biochimie et biologie cellulaire, 72(5-6), 239-243 (1994-05-01)
The major advantages of the horseradish peroxidase chemiluminescence (HRP-CL) immunodetection method in Western blot analysis are its high sensitivity, nonradioactive detection, economy of the primary antibody, and speed of detecting the signal. However, we observed a strong and reproducible signal
Raul Bettencourt et al.
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 152(2), 278-289 (2008-12-02)
The interaction between microorganisms and host defense mechanisms is a decisive factor for the survival of marine bivalves. They rely on cell-mediated and humoral reactions to overcome the pathogens that naturally occur in the marine environment. In order to understand
K A Brogden et al.
Infection and immunity, 67(8), 4256-4259 (1999-07-23)
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Protocols

Gelatin Blocking Buffer for Direct Detection Southern or Western Blotting

In order to specifically detect an antigen or target molecule immobilized on a solid support, unoccupied binding sites on the support must be blocked against binding by probe and detection molecules.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Northern和Southern印迹实验方案及简介

Northern和Southern印迹简介：两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。

用于直接检测型Southern或Western Blotting的明胶封闭缓冲液

为了特异性地检测固定在固体支持物上的抗原或靶分子，必须将支持物上未被占据的结合位点进行封闭以防止探针和检测分子结合。

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