Key Documents

View All Documentation

L2542

LB Broth (Miller)

Name: LB Broth (Miller)
Brand: SIGMA

Liquid microbial growth medium

Synonym(s):

Luria Broth Miller, Luria-Bertani broth, Miller

Select a Size

Change View

About This Item

NACRES:

NA.85

UNSPSC Code:

41106200

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

grade

Molecular Biology

Quality Level

400

sterility

sterile; 0.2 μm filtered

form

liquid

technique(s)

microbiological culture: suitable

pH

6.5-7.2

application(s)

microbiology

storage temp.

room temp

suitability

nonselective for Escherichia coli, nonselective for coliforms

Looking for similar products? Visit Product Comparison Guide

Related Categories

Cloning & Expression

Industrial Microbiology

Microbial Culture Media

Molecular Cloning & Protein Expression

General description

Miller’s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates. It enables fast growth, improves growth yields for various bacterial species, and can retain the hydrophilic behavior of samples for longer periods.

Application

LBBroth (Miller) is suitable for:

non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins.
selective cultivation when appropriate antibiotics are added.
the cultivation of E-coli cells for anti-bacterial assays.

Features and Benefits

Miller′s LB liquid provides:

Filter-sterilized, ready-to-use format
Convenient package size
Standard formulation

Other Notes

10g/L Tryptone
10 g/L NaCl
5 g/L Yeast Extract

Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Information

动物来源培养基

This item has

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Use of the Yeast Pichia pastoris as an Expression Host for Secretion of Enterocin L50, a Leaderless Two-Peptide (L50A and L50B) Bacteriocin from Enterococcus faecium L50

Antonio Basanta, Beatriz Gomez-Sala, et al.

Biotechnology, 76, 3314-3324 (2010)

Long-term retention of hydrophilic behavior of plasma treated polydimethylsiloxane (PDMS) surfaces stored under water and Luria-Bertani broth

Zhao LH, et al.

Sensors and actuators A, Physical, 181.0, 33-42 (2012)

Quaternary ammonium N,N,N-trimethyl chitosan derivative and povidone‑iodine complex as a potent antiseptic with enhanced wound healing property.

Celine Abueva et al.

International journal of biological macromolecules, 182, 1713-1723 (2021-05-30)

The importance of developing more potent antimicrobials and robust infection prevention practices has been highlighted recently with the increase in reports of emerging bacterial resistance mechanisms and the development of antibiotic-resistant microbes. In this study, a quaternary ammonium chitosan derivative

Grainyhead-Like 3 Influences Migration and Invasion of Urothelial Carcinoma Cells.

Felix Wezel et al.

International journal of molecular sciences, 22(6) (2021-04-04)

Invasive urothelial carcinomas of the bladder (UCB) characteristically show a loss of differentiation markers. The transcription factor Grainyhead-like 3 (GRHL3) plays an important role in the development and differentiation of normal urothelium. The contribution to UCB progression is still elusive.

Acaricidal activities of whole cell suspension, cell-free supernatant, and crude cell extract of Xenorhabdus stokiae against mushroom mite (Luciaphorus sp.).

Prapassorn Bussaman et al.

Journal of Zhejiang University. Science. B, 13(4), 261-266 (2012-04-03)

Xenorhabdus bacterium has been used as a biological control agent against Luciaphorus sp., a mushroom mite endemic in Thailand. To develop an effective formulation of Xenorhabdus stokiae, treatments using different parts of X. stokiae isolate PB09 culture, including whole cell

View All Related Papers

Articles

Protein Expression Systems

Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.

蛋白质表达系统

基因工程和克隆的发展为研究领域开辟了表达和分离异源蛋白质的许多可能性。技术上巨大进步使得能够大规模表达和分离重组蛋白。

Protocols

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

微生物生长实验方案

在从小型培养到发酵级的不同规模下培养大肠杆菌。包括了用于启动起始培养物、悬浮细胞、单一培养物和平板噬菌体M13的实验方案。

Global Trade Item Number

SKU	GTIN
L2542-500ML	04061833950180

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service