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P8804

Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus

Name: Phospholipase C, Phosphatidylinositol-specific from <I>Bacillus cereus</I>
Brand: SIGMA

buffered aqueous glycerol solution, ≥1,000 units/mg protein (Lowry)

Synonym(s):

1-Phosphatidyl-D-myo-inositol inositol phosphohydrolase, cyclic-phosphate forming, PI-PLC

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About This Item

CAS Number:

37288-19-0

UNSPSC Code:

12352204

NACRES:

NA.32

EC Number:

232-638-2

MDL number:

MFCD00131896

EC Number:

4.6.1.13 (BRENDA, IUBMB)

Specific activity:

≥1,000 units/mg protein (Lowry)

Biological source:

Bacillus sp. (Bacillus cereus)

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biological source

Bacillus sp. (Bacillus cereus)

Quality Level

200

form

buffered aqueous glycerol solution

specific activity

≥1,000 units/mg protein (Lowry)

mol wt

28 kDa

foreign activity

Phopholipase C (lecithinase) ≤1 units/mg protein, Sphingomyelinase ≤40 units/mg protein

storage temp.

2-8°C

Gene Information

Bacillus cereus E33L ... BCZK3513(3026815)

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General description

Phospholipase C (PI-PLC) from Bacillus cereus has irregular triosephosphate isomerase (TIM)-barrel structure with eight-standard parallel β barrel. It is a 28 kDa protein.

Application

Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus has been used:

in the hydrolysis of substrates p-nitrophenylphosphorylcholine (p-NPPC) and p-nitrophenylphosphorylphosphate (p-NPP)
to cleave glycosylphosphatidylinositol (GPI) anchor of lynx1 protein and its detachment from plasma membrane
to cleave immunolabeled HeLa cells

Biochem/physiol Actions

Phospholipase C (PI-PLC) releases diacylglycerol by cleaving the glycosylphosphatidylinositol. It has broad substrate specificity and its activity is influenced by metal ions and surfactants.

Used for the release of GPI anchored proteins from the membrane.

Physical form

Solution in 60% (v/v) glycerol containing 10 mM Tris-HCl, pH 8.0 and 10 mM EDTA

Analysis Note

Acetylcholinesterase is measured according to Ellman, et al.

Other Notes

One unit will liberate one unit of acetylcholinesterase per minute from a membrane-bound crude preparation at pH 7.4 at 30 °C (10 minute incubation).

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number

0000469923

0000427386

0000119053

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Ligand binding characteristics of a glycosylphosphatidyl inositol membrane-anchored HeLa cell folate receptor epitope-related to human milk folate binding protein

Holm J, et al.

Bioscience Reports, 20(2), 109-118 (2000)

Crystal structure of phosphatidylinositol-specific phospholipase C from Bacillus cereus in complex with glucosaminyl (alpha1? 6)-d-myo-inositol, an essential fragment of GPI anchors

Heinz DW, et al.

Biochemistry, 35(29), 9496-9504 (1996)

High-level expression of recombinant phospholipase C from Bacillus cereus in Pichia pastoris and its characterization

Seo KH and Rhee JI

Biotechnology Letters, 26(19), 1475-1479 (2004)

Lynx1 shifts alpha4beta2 nicotinic receptor subunit stoichiometry by affecting assembly in the endoplasmic reticulum

Nichols WA, et al.

The Journal of Biological Chemistry, 289(45), 31423-31432 (2014)

Release of alkaline phosphatase from membranes by a phosphatidylinositol-specific phospholipase C.

M G Low et al.

The Biochemical journal, 167(1), 281-284 (1977-10-01)

Purified phosphatidylinositol-specific phospholipase C from Staphylococcus aureus released a substantial proportion of the total alkaline phosphatase activity from a wide range of tissues from several mammalian species. Co-purification of the phospholipase C and alkaline phosphatase-releasing activities and the inhibition of

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Glycosylphosphatidylinisotol-anchored Glycoproteins Overview

Glycosylphosphatidylinisotol- (GPI) anchored proteins have been identified throughout a broad range of eukaryotic species ranging from humans to insects, yeasts, bacteria, and fungi, suggesting that they are an ancient modification.

糖基化磷脂酰肌醇锚定糖蛋白概述

GPI锚定糖蛋白

Global Trade Item Number

SKU	GTIN
P8804-5UN	04061832856865
P8804-25UN	04061832856858

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