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S0389

Sigma-Aldrich

Sucrose

for molecular biology, ≥99.5% (GC)

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Synonym(s):
α-D-Glc-(1→2)-β-D-Fru, α-D-Glucopyranosyl β-D-fructofuranoside, β-D-Fructofuranosyl-α-D-glucopyranoside, D(+)-Saccharose, Sugar
Empirical Formula (Hill Notation):
C12H22O11
CAS Number:
57-50-1
Molecular Weight:
342.30
Beilstein:
90825
EC Number:
200-334-9
MDL number:
MFCD00006626
PubChem Substance ID:
24899423
NACRES:
NA.31

biological source

sugar cane

Quality Level

300

grade

for molecular biology

Assay

≥99.5% (GC)

form

crystals

technique(s)

HPLC: suitable
cryopreservation: suitable
electron microscopy: suitable
enzyme immunoassay: suitable
gas chromatography (GC): suitable
immunohistochemistry: suitable

impurities

≤0.1% free glucose

color

white

useful pH range

5.5-7 (25 °C, 342 g/L)

mp

185-187 °C (lit.)

solubility

H2O: soluble 50 g + 50 mL

cation traces

heavy metals (as Pb): ≤5 ppm

absorption

≤0.15 at 280 at 50%
≤0.20 at 260 at 50%

suitability

suitable for molecular biology

application(s)

agriculture
agriculture
life science and biopharma
metabolomics
sample preservation

foreign activity

DNase, RNase, protease, none detected

storage temp.

room temp

SMILES string

OC[C@H]1O[C@H](O[C@]2(CO)O[C@H](CO)[C@@H](O)[C@@H]2O)[C@H](O)[C@@H](O)[C@@H]1O

InChI

1S/C12H22O11/c13-1-4-6(16)8(18)9(19)11(21-4)23-12(3-15)10(20)7(17)5(2-14)22-12/h4-11,13-20H,1-3H2/t4-,5-,6-,7-,8+,9-,10+,11-,12+/m1/s1

InChI key

CZMRCDWAGMRECN-UGDNZRGBSA-N

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General description

Sucrose, a disaccharide composed of glucose and fructose, serves as an energy source in various biological processes. In cell biology, sucrose acts as a cryoprotectant, preserving cell viability during freezing and thawing. It is also added as a supplement in culture media, aiding in maintaining osmotic balance and enhancing cell survival.In molecular biology, sucrose is crucial in gradient solutions for DNA and RNA purification and in density centrifugation for organelle separation.

Application

Sucrose has been used:
  • to perform cryoprotection for kidney tissues
  • as a component in the solid germination medium to germinate pollen grains
  • in the triphenyl-tetrazolium chloride (TTC) test to check the viability of pollen grains
  • in the cryoprotection of fixed brain tissues for immunohistochemistry

Biochem/physiol Actions

Sucrose is used as a transport and storage molecule in most plants due to its limited chemical reactivity. It can be used to prepare density gradients for the separation of cell/organelle. Sucrose can be used in the plant, insect, and bacterial culture media as a supplement. In addition, sucrose can also be used in several enzymatic assays.

Features and Benefits

  • Ideal for Molecular Biology, Cell Biology and Biochemical research
  • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
  • Tested for DNase, RNase, and proteases
  • Molecular biology grade sucrose, sourced from sugar cane

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
For additional information on our range of Biochemicals, please complete this form.
To gain a comprehensive understanding of our extensive range of Disaccharides for your research, we encourage you to visit our Carbohydrates Category page.

comparable product

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

监管及禁止进口产品

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Julia B Cordero et al.
The EMBO journal, 33(13), 1474-1491 (2014-05-03)
The non-receptor tyrosine kinase c-Src, hereafter referred to as Src, is overexpressed or activated in multiple human malignancies. There has been much speculation about the functional role of Src in colorectal cancer (CRC), with Src amplification and potential activating mutations
Denise Harrison et al.
Pediatrics, 130(5), 918-925 (2012-10-10)
The use of oral sucrose has been the most extensively studied pain intervention in newborn care to date. More than 150 published studies relating to sweet-taste-induced calming and analgesia in human infants have been identified, of which 100 (65%) include
Rafaela Ribeiro Reis et al.
Plant science : an international journal of experimental plant biology, 221-222, 59-68 (2014-03-25)
Drought is one of the most challenging agricultural issues limiting sustainable sugarcane production and, in some cases, yield losses caused by drought are nearly 50%. DREB proteins play vital regulatory roles in abiotic stress responses in plants. The transcription factor
Zsuzsanna Tucsek et al.
The journals of gerontology. Series A, Biological sciences and medical sciences, 69(10), 1212-1226 (2013-11-26)
There is growing evidence that obesity has deleterious effects on the brain and cognitive function in the elderly population. However, the specific mechanisms through which aging and obesity interact to promote cognitive decline remain unclear. To test the hypothesis that
Marcus Karlstetter et al.
Progress in retinal and eye research, 45, 30-57 (2014-12-06)
Resident microglial cells can be regarded as the immunological watchdogs of the brain and the retina. They are active sensors of their neuronal microenvironment and rapidly respond to various insults with a morphological and functional transformation into reactive phagocytes. There
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