SHC003
MISSION® pLKO.1-puro-CMV-TurboGFP™ Positive Control Plasmid DNA
Green fluorescent protein marker to monitor transduction efficiency
Quality Level
product line
MISSION®
concentration
500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)
shipped in
dry ice
storage temp.
−20°C
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General description
Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The TurboGFP Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.
Application
Legal Information
recommended
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Regulatory Information
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
What competent cells can I use to propagate the MISSION® Control Vector Purified DNA TurboGFP™ plasmid, Product SHC003?
We use GC5 cells (identical genotype to DH5α-T1R) to propagate the Mission shRNA plasmids and controls.
How much plasmid DNA do I receive when I order MISSION® Control Vector Purified DNA TurboGFP™, Product SHC003?
Each MISSION Control Vector is provided as 10 μg purified plasmid DNA at a concentration of ~500 ng/μL in 10 mM Tris-HCl, pH 8.0, containing 1 mM EDTA.
Can I use MISSION® Control Vector Purified DNA TurboGFP™ plasmid, Product SHC003, to produce lentiviral particles?
The MISSION® Lentiviral Packaging Mix can be used if you wish to produce virus in your own lab. The product code is SHP001. This product is an optimized formulation of two plasmids expressing the key HIV packaging genes and a heterologous viral envelope gene. The Lentiviral Packaging Mix is designed to be co-transfected along with a compatible lentiviral transfer vector (such as SHC003) into HEK293T cells in order to create high-titer pseudo-typed lentiviral particles used for downstream transduction applications. The procedure is available in the Technical Bulletin for product SHP001.
Can I purchase lentiviral particles produced using MISSION® Control Vector Purified DNA TurboGFP™ plasmid, Product SHC003, directly from Sigma-Aldrich?
Yes. The product code is SHC003V.
What other MISSION® shRNA controls are available from Sigma-Aldrich?
A complete list of our MISSION® controls can be found here.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Articles
When shRNA is delivered using lentiviral vectors, the sequence encoding the shRNA is integrated into the genome and the knockdown effect is passed on to daughter cells, continuing gene silencing.
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