T8787
Triton™ X-100
Molecular Biology
Synonym(s):
t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether
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About This Item
InChI
1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3
SMILES string
CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1
InChI key
HNLXNOZHXNSSPN-UHFFFAOYSA-N
biological source
synthetic (organic)
grade
Molecular Biology
description
non-ionic
form
liquid
mol wt
micellar avg mol wt 80,000, average mol wt 625
Quality Level
technique(s)
protein purification: suitable, protein quantification: suitable, western blot: suitable
impurities
<1.00% water (Karl Fischer)
pH
9.7
CMC
0.2-0.9 mM (20-25°C)
mp
6 °C
transition temp
pour point ~7 °C
solubility
water: 0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow
density
1.06 g/mL at 25 °C (lit.)
cation traces
Fe: <5 ppm, K: <0.05%, Na: <0.1%, heavy metals: <5 ppm
application(s)
hematology
histology
foreign activity
DNase and RNase, none detected
storage temp.
room temp
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General description
Application
- In immunohistochemistry for staining the Flat-mount retinas
- Along with ice-cold PBS (phosphate buffered saline) in suspension of cells for cell DNA analysis and Annexin V assay
- To permeabilise cells during Immunofluorescent microscopic studies
- As a positive control in LDH assay to determine the cell membrane integrity
- For estimating the lipase activity in postheparin plasma by using modified Belfrage and Vaughan radioenzymatic procedure
- For the preparation of outer membrane protein exctract
- As a component of extraction buffer along with tris-HCl, NaCl, CaCl2, ZnCl2, Brij 35 for homogenization of mice lung cells
- In the treatment of tissue sections for Immunofluorescence labeling
Biochem/physiol Actions
Features and Benefits
- Ideal for sensitive molecular biology research applications
- Tested for DNase and RNase
- Non-ionic nondenaturing detergent
- Superior wetting agent and emulsifier
- Highly versatile surfactant
Other Notes
Legal Information
signalword
Danger
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
483.8 °F - closed cup
flash_point_c
251 °C - closed cup
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - ED ENV 1 - Eye Dam. 1 - Skin Irrit. 2
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