fluorescence
λex 552 nm;λem 578 nm
manufacturer/tradename
AAT Bioquest
General description
As native casein this rhodaminated casein is hydrolyzed by many proteases, and widely used for fluorometric measurement of protease activity. In the intact substrate, casein is heavily labeled with TAMRA, resulting in significant fluorescence quenching. Protease-catalyzed hydrolysis relieves its quenching effect, yielding brightly orange fluorescent dye-labeled short peptides. The increase in fluorescence intensity is directly proportional to protease activity.
Casein, TAMRA-conjugated is manufactured by AAT Bioquest
Casein, TAMRA-conjugated is manufactured by AAT Bioquest