AAT Bioquest Protonex™ Red 780 AM

Protonex™ Red 780 AM is the cell-permeable version of Protonex™ Red 780. Protonex™ Red 780 is minimally fluorescent at a basic pH and maximally fluorescent at an acidic pH. When Protonex™ Red 780 is bound to a receptor or an antibody on the cell surface, it is essentially non-fluorescent because the extracellular pH is neutral. However, when the receptor or antibody is internalized into the cell in response to a stimulus, it enters the endosomal pathway, where the pH is acidic. This causes Protonex™ Red 780 to become highly fluorescent and emit near-infrared light when excited by a red laser. By measuring the fluorescence intensity of Protonex™ Red 780, one can monitor the activation and trafficking of receptors or antibodies in live cells.

Protonex™ Red 780 AM *Cell-Permeable* is manufactured by AAT Bioquest

Protonex™ is a trademark of AAT Bioquest