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UNSPSC Code:
12141717
NACRES:
NA.22
Quality Level
description
Ni-NTA surface density: 1/nm2
concentration
in water
OD
50
core diam.
10 nm , coefficient of variance <12%
diameter
10 nm
λmax
520 nm
PDI
<0.200
functional group
Ni-NTA
storage temp.
2-8°C
InChI
1S/Au
InChI key
PCHJSUWPFVWCPO-UHFFFAOYSA-N
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General description
Ni-NTA functionalized gold nanoparticles are suitable for binding of proteins with a C-, or N-terminal 6X His tag. These gold nanoparticles allow for rapid generation of gold conjugates for subsequent use in downstream assays. Ni-NTA functionalized gold nanoparticles are more than 95% spherical and have uniform size distribution (CV <12%).
Application
Ideal for development of gold conjugates for use in applications such as blotting, lateral flow assays, microscopy, and transmission electron microscopy (TEM).
Preparation Note
When stored for a long period of time gold nanoparticles may sediment at the bottom of the vial, which is especially prominent for larger particle sizes. Prior to use, re-suspend the sedimented particles by thorough mixing until a homogenous solution is obtained.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Regulatory Information
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A generic approach to monofunctionalized protein-like gold nanoparticles based on immobilized metal ion affinity chromatography.
Raphaël Lévy et al.
Chembiochem : a European journal of chemical biology, 7(4), 592-594 (2006-02-14)
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