937991
Protein delivery system
Human FGF-20, PODS®
Synonym(s):
PODS®
biological source
(Spodoptera frugiperda (Sf9) cell culture)
form
(monodisperse crystals)
packaging
1 ea of 25 μg
technique(s)
: suitable using 3D bioprinting | cell culture
impurities
<0.06 EU/mL Endotoxin
color
colorless
storage temp.
2-8°C
Quality Level
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General description
Protein delivery system, Human FGF-20, PODS polyhedrin protein co-crystalized with Human FGF-20. Fibroblast Growth Factor 20 (FGF-20) is a member of the fibroblast growth factor family, more specifically of the FGF-9 subfamily based on their structural and sequence similarities. It is expressed by a variety of cell types including fibroblasts, dopaminergic neurons, keratinocytes and epithelial cells. FGF-20, also regarded as a secreted neurotrophic factor, is involved in many biological processes, such as tumour growth, tissue repair, and embryonic development, specifically in the development of the central nervous system. It has shown to interact with FGF receptors 1c, 2c, 3b and 3c and 4c.
Application
PODS are composed of a bioactive cargo protein within a polyhedrin crystal lattice, with polyhedrin as the main protein. The 25 µg SKU size indicates the amount of bioactive cargo, typically 0.5% to 5% of the total protein. For instance, a 25 µg SKU at a 2% cargo loading contains 1.25 mg of total protein. PODS enable sustained release of cargo proteins when proteases degrade the polyhedrin scaffold. Growth factors are not bioavailable until released, and their concentration in media depends on the amount added and the release rate. In 10% serum, peak levels are usually reached within 24-48 hours, with about 20% of the cargo becoming soluble.
PODS often require lower concentrations than conventional growth factors, as they maintain effective levels. Pre-incubating with serum or proteases enhances availability, while conventional factors can be added for immediate effects. Optimal amounts should be determined empirically.
PODS often require lower concentrations than conventional growth factors, as they maintain effective levels. Pre-incubating with serum or proteases enhances availability, while conventional factors can be added for immediate effects. Optimal amounts should be determined empirically.
Features and Benefits
- Protein delivery system, PODS Human FGF-20 comes with molecular weight of 28.6 kDa, length 256 aa and endotoxin level <0.06 EU/ml. PODS co-crystals may be reconstituted at 200 million co-crystals/ml in sterile PBS. 20% glucose has a buoyant density closer to PODS co-crystals and can be useful for aliquoting.
- This product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols. PODS co-crystals are highly stable when stored in aqueous solution (pH range 6 - 8).
- PODS provides durability, stability AND sustained release, addressing the limitations of conventional and enabling researchers to effortlessly control and manipulate cells in 2D and 3D culture as well as in-vivo.
Legal Information
PODS is a registered trademark of Cell Guidance Systems Ltd
Storage Class
11 - Combustible Solids
Regulatory Information
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