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939277

Sigma-Aldrich

Protein delivery system

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Human TNF-α, PODS®

Synonym(s):

PODS®

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About This Item

biological source

(Spodoptera frugiperda (Sf9) cell culture)

Quality Level

100

form

(monodisperse crystals)

packaging

1 ea of 25 μg

technique(s)

: suitable using 3D bioprinting | cell culture

impurities

<0.06 EU/mL Endotoxin

color

colorless

storage temp.

2-8°C

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General description

Protein delivery system, TNF-α, PODS contains the polyhedrin protein co-crystalized with Human TNF-α. Tumor Necrosis Factor alpha (TNF-α) is a prototypic ligand of the TNF superfamily, a pleiotropic pro-inflammatory cytokine secreted by various cells, including adipocytes, monocytes, macrophages, B cells, T cells, fibroblasts, and tumor cells. Thus, playing an important role in inflammation, immune system development, apoptosis, and lipid metabolism. There is significant cross-species reactivity between human and mouse cytokines.

Application

PODS are composed of a bioactive cargo protein within a polyhedrin crystal lattice, with polyhedrin as the main protein. The 25 µg SKU size indicates the amount of bioactive cargo, typically 0.5% to 5% of the total protein. For instance, a 25 µg SKU at a 2% cargo loading contains 1.25 mg of total protein. PODS enable sustained release of cargo proteins when proteases degrade the polyhedrin scaffold. Growth factors are not bioavailable until released, and their concentration in media depends on the amount added and the release rate. In 10% serum, peak levels are usually reached within 24-48 hours, with about 20% of the cargo becoming soluble.

PODS often require lower concentrations than conventional growth factors, as they maintain effective levels. Pre-incubating with serum or proteases enhances availability, while conventional factors can be added for immediate effects. Optimal amounts should be determined empirically.

Features and Benefits

  • Protein delivery system, PODS human TNF-α comes with molecular weight of 40.7 kDa, length 332 aa and endotoxin level <0.06 EU/ml. PODS co-crystals may be reconstituted at 200 million co-crystals/ml in sterile PBS. 20% glucose has a buoyant density closer to PODS co-crystals and can be useful for aliquoting.
  • This product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols. PODS co-crystals are highly stable when stored in aqueous solution (pH range 6 - 8).
  • PODS provides durability, stability and sustained release, addressing the limitations of conventional and enabling researchers to effortlessly control and manipulate cells in 2D and 3D culture as well as in-vivo.

Legal Information

PODS is a registered trademark of Cell Guidance Systems Ltd

Storage Class Code

11 - Combustible Solids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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