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Merck
CN

940836

TissueFab® PODS Growth Factor Loaded Bioink Kit

Cartilage differentiation, Facile curable

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description

Application(s): cartilage differentiation, cell culture, tissue engineering, Bioburden (Total Aerobic): <5 CFU/ml, Bioburden (fungal): < 5 CFU/mL, Endotoxin: <50 EU/ml

form

viscous liquid

composition

PODS Human IGF-1, 25µg , PODS Human TGFb-1, 25µg , PODS Human TGFB-2, 25µg , TissueFab® bioink, Facile Curable GelHA, Ionic

color

colorless to yellow

compatibility

(extrusion-based 3D bioprinting)

storage temp.

−20°C

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General description

Rethink your bioinks with TissueFab®

About the material:

The TissueFab® PODS Growth Factor Loaded Bioink Kit, Cartilage differentiation, Facile Curable contains:

  • Low endotoxin gelatin-hylauronic acid based bioink
  • Human IGF-1 PODS
  • Human TGFβ-1 PODS
  • Human TGFβ-2 PODS
Printing and crosslinking parameters:

  • Suitable for extrusion-based bioprinting
  • Ionic crosslinking
TissueFab® PODS Growth Factor Loaded Bioink kit, Cartilage differentiation, Facile curable is a ready-to-use bioink kit which is formulated for high cell viability, printing fidelity, and is designed for extrusion-based 3D bioprinting. The bioink component has been formulated to crosslink without the necessity of light irradiation which can be detrimental to cell health. It contains a Gelatin base which as a natural protein offers excellent cytocompatibility and cell-binding motifs in order to mimic the native extracellular (ECM) microenvironment. The bioink can be crosslinked in a dilute solution of multivalent cations including TissueFab(R) - crosslinking solution (Cat#919926). TissueFab® - Facile curable HA bioink also contains hyaluronic acid (HA), a natural polysaccharide found ubiquitously thorughout the body and cellular ECM. Cell can recognize HA through cell surface receptors CD44 and RHAMM and also degrade it through secreation of hyaluronidase TissueFab® PODS Growth Factor Loaded Bioink kit, Cartilage differentiation, Facile curable can be used with most extrusion-based bioprinters, is biodegradable, and is compatible with human mesenchymal stem cells (hMSCs). Included growth facors, IGF-1, TGFb-1, and TGFb-2, are known to play crucial roles in chondrogenic differentiation, proliferation, and repair. Their inclusion with PODS (R) delivery system allows their sustained release within bioprinted constructs and retainment of 70% bioactivity after 6 months. When mixed with the bioink component, the included PODS (R) growth factors can promote chondrogenic differentiation of encapsulated cells.

More information and a step by step protocol can be found under the "More Documents" section of this page.

Application

TissueFab® PODS Growth Factor Loaded Bioink kit, Cartilage differentiation, facile curable enables the precise fabrication of chondrogenic 3D cell models and tissue constructs for research in 3D cell biology, tissue engineering, in vitro tissue models, and regenerative medicine.

Features and Benefits

Key Features and Benefits:

Each TissueFab® bioink is developed and tested to meet strict criteria to best suit researchers′ needs. This includes:

  • Printability and rheological properties
  • Crosslinking
  • Cell viability
  • Biocompatibility
  • Low bioburden
  • Low endotoxin
This product contains 10 ml of bioink solution and growth factors PODS® packaged separately to allow for optimization.

Legal Information

PODS is a registered trademark of Cell Guidance Systems Ltd
TISSUEFAB is a registered trademark of Merck KGaA, Darmstadt, Germany

Kit Components Also Available Separately

Product No.
Description
SDS
  • 938076Protein delivery system, Human IGF-1, PODS®SDS
  • 930016TissueFab® bioink  , Facile Curable GelHA, IonicSDS
  • 939234Protein delivery system, Human TGF-β1, PODS®SDS
  • 939242Protein delivery system, Human TGF-β2, PODS®SDS

Storage Class

10 - Combustible liquids

Regulatory Information

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Certificates of Analysis (COA)

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Hyaluronan: a simple polysaccharide with diverse biological functions
Dicker K T, et al.
Acta Biomaterialia, 10(4), 1558-1570 (2014)
Insulin-like growth factor-1 in articular cartilage repair for osteoarthritis treatment
Wen C, et al.
Arthritis Research & Therapy, 23(1), 277-277 (2021)
The role of TGF-?2 in cartilage development and diseases
Duan M, et al.
Bone & joint research, 10(8), 474-487 (2021)
A single day of TGF-?1 exposure activates chondrogenic and hypertrophic differentiation pathways in bone marrow-derived stromal cells
Futrega K, et al.
Communications biology, 29, 4(1)-4(1) (2021)

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