DCA Deblock

Name: DCA Deblock
Brand: ALDRICH

with Dichloromethane

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About This Item

NACRES:

NA.21

UNSPSC Code:

41116004

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Properties

Quality Level

200

type

for DNA synthesis

product line

Proligo Reagents

form

liquid

storage condition

dry at room temperature

concentration

42.2-51.6 g/L (DCA content, titration)

impurities

≤200 ppm water content (Karl Fischer)

color

clear

Description

General description

Deblock solution contains dichloroacetic acid (DCA) in dichloromethane that assists in the removal of the dimethoxytrityl (DMT) protecting group from the 5′hydroxyl moiety of nucleotides already incorporated into the growing nucleic acid, before the addition of the next phosphoramidite. Removal of the DMT allows the unprotected 5′hydroxyl moiety to react with a new phosphoramidite in a subsequent extension reaction.

Preparation Note

Dicloromethane / Dichloroacetic acid 97/3 (v/v)

Other Notes

Deblock solution containing dichloroacetic acid (DCA) in dichloromethane removes the dimethoxytrityl (DMT) protecting group from the 5′hydroxyl moiety of nucleotides already incorporated into the growing nucleic acid, prior to the addition of the next phosphoramidite. Removal of the DMT allows the unprotected 5′hydroxyl moiety to react with a new phosphoramidite in a subsequent extension reaction.