ETT Activator

Name: ETT Activator
Brand: ALDRICH

0.25 M, configured for PerkinElmer, configured for Polygen, configured for MerMade, configured for ABI 3900

Synonym(s):

5-(Ethylthio)-1H-tetrazole, 5-(Ethylthio)tetrazole solution

Select a Size

Change View

About This Item

NACRES:

NA.21

PubChem Substance ID:

329817352

UNSPSC Code:

41116105

MDL number:

MFCD00068733

Form:

liquid

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

type

for DNA synthesis

product line

Proligo Reagents

form

liquid

storage condition

dry at room temperature

concentration

32-35 g/L (ETT content, titration)

impurities

≤30 ppm water content (Karl Fischer)

color

clear

compatibility

configured for ABI 3900, configured for MerMade, configured for PerkinElmer, configured for Polygen

SMILES string

CCSc1nnn[nH]1

InChI

1S/C3H6N4S/c1-2-8-3-4-6-7-5-3/h2H2,1H3,(H,4,5,6,7)

InChI key

GONFBOIJNUKKST-UHFFFAOYSA-N

Description

Preparation Note

Acetonitrile / 5-(Ethylthio)-1H-Tetrazole 100 / 3 (v/w)

Other Notes

Activator solution, containing 5-(Ethylthio)-1H- tetrazole (ETT) in acetonitrile, are mixed with solutions of phosphoramidites during the extension step.
The activator reacts with the amidite group to form a highly reactive intermediate. The intermediate then forms an internucleotide bond with the detritylated 5′hydroxyl group of the growing oligonucleotide chain.