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L040040

Sigma-Aldrich

Cap A

configured for MerMade

Quality Level

type

for DNA synthesis

product line

Proligo Reagents

assay

97.0-119.0 g/L (Acetic Anhydride content, titration)

shelf life

365 days

color

clear colorless, to brownish

compatibility

configured for MerMade

storage temp.

2-8°C

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Preparation Note

Tetrahydrofurane / Lutidine / Acetic Anhydride 8/1/1 (v/v/v)

Other Notes

Cap A solution, containing acetic anhydride, lutidine and tetrahydrofuran (THF), is used after the phosphoramidite reaction/coupling step. This solution aborts chains bearing unreacted 5′hydroxyl groups due to failure of reaction with activated phosphoramidite (typically, 1 to 2% of growing oligonucleotide chains in each synthesis cycle). These unreacted 5′hydroxyl groups are capped with an acetyl group and thus rendered unreactive for subsequent synthesis steps.

Certificate of Analysis

Certificate of Origin

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