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Avanti

E. coli Extract Polar

Avanti Polar Lipids

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Synonym(s):
E. coli Polar Lipid Extract
CAS Number:
1240502-50-4
NACRES:
NA.25

form

powder

packaging

pkg of 1 × 1 g (100600P-1g)
pkg of 1 × 100 mg (100600P-100mg)
pkg of 5 × 100 mg (100600P-500mg)

manufacturer/tradename

Avanti Polar Lipids

lipid type

lipid extracts

shipped in

dry ice

storage temp.

−20°C

General description

This product is an extract of E. coli B (ATCC 11303) grown in Kornberg Minimal media at 37°C and taken at 3/4 log growth phase. Total E. coli lipid extract is a chloroform:methanol extract of the respective tissue. This extract is partitioned against deionized water and the chloroform phase is concentrated. Polar lipid extract is the total lipid extract precipitated with acetone and then extracted with diethyl ether.

Application

E. coli Extract Polar has been used for the preparation of lipid standards for validation purposes. It has also been used for determining the molar percentages of lipids.
E. coli Extract Polar has been used:
  • in liposome preparation for spectroscopy studies using fluorescent membrane dye, laurdan
  • in multilamellar vesicles generation
  • as standard lipid material for tandem mass spectrometry analysis

E. coli Extract Polar is suitable: to prepare lipid standard material by dissolving in methanol to prepare small unilamellar vesicle to prepare liposomes (in Tris-hydrochloric acid (HCl)

Biochem/physiol Actions

E. coli Polar lipid extracts are useful for reconstituting membrane proteins. The phosphatidylethanolamine is the predominant lipid component of E. coli extract polar extract. The minor components include the phosphatidylglycerol (PG) and cardiolipin. These lipids are majorly derived from the leaflets of cytoplasmic membrane and inner leaflet of outer membrane. E. coli polar lipid extracts form bilayer by vesicle fusion.

Packaging

5 mL Clear Glass Sealed Ampule (100600P-100mg)
5 mL Clear Glass Sealed Ampule (100600P-500mg)
60 mL Amber Wide Mouth Screw Cap Glass Bottle (100600P-1g)

Storage Class Code

11 - Combustible Solids

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Using supported bilayers to study the spatiotemporal organization of membrane-bound proteins
Methods in Cell Biology, 128, 223-241 (2015)
Molecular model for the solubilization of membranes into nanodisks by styrene maleic acid copolymers
Scheidelaar S, et al.
Biophysical Journal, 108(2), 279-290 (2015)
Assessing Membrane Fluidity and Visualizing Fluid Membrane Domains in Bacteria Using Fluorescent Membrane Dyes
Wenzel M, et al.
Bio-protocol, 8(20) (2018)
Assessing membrane fluidity and visualizing fluid membrane domains in bacteria using fluorescent membrane dyes
Wenzel M, et al.
Bio-protocol (2018)
Emily Peterson et al.
Biochimica et biophysica acta, 1808(2), 516-521 (2010-10-26)
Amantadine-sensitive proton uptake by liposomes is currently the preferred method of demonstrating M2 functionality after reconstitution, to validate structural determination with techniques such as solid-state NMR. With strong driving forces (two decades each of both [K(+)] gradient-induced membrane potential and
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