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Merck
CN

1.04227

Methyl green zinc chloride double salt (C.I. 42590)

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About This Item

Empirical Formula (Hill Notation):
C27H35BrClN3 · ZnCl2
CAS Number:
7114-03-6
Molecular Weight:
653.24
NACRES:
NA.21
UNSPSC Code:
41116130
MDL number:
MFCD00151094

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InChI

1S/C27H35N3.4ClH.Zn/c1-8-30(6,7)26-19-13-23(14-20-26)27(21-9-15-24(16-10-21)28(2)3)22-11-17-25(18-12-22)29(4)5;;;;;/h9-20H,8H2,1-7H3;4*1H;/q+2;;;;;+2/p-4

InChI key

ZKOGXCSOLMZRAZ-UHFFFAOYSA-J

form

solid

composition

Dye, ≥50% spectrophotometric assay

pH

4.6 (20 °C, 10 g/L in H2O)

mp

>300 °C

solubility

70 g/L

bulk density

300 kg/m3

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-30°C

Quality Level

200

General description

Methyl green zinc chloride double salt (C.I. 42590) for microscopy Certistain®, is a dry dye that is used for the preparation of a staining solution e.g. for DNA and RNA staining in methyl green-pyronine G staining method in histological sections of human origin. As a CERTISTAIN® dye it is chemically quality-certified according to highest standards and strict specifications.

Application

for microscopy Certistain®

Analysis Note

Dye content (spectrophotometrically): ≥ 50 % C₂₇H₃₅BrClN₃
Identity
- Bromide:
passes test
- chloride: passes test
- Zinc: passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ max (water): 630 - 635 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.005 g/l; water): ≥ 810
Crystal violet: ≤ 4 %
TLC-Test: passes test
Loss on drying (50 ° C/0.05 mm Hg/ 3h): ≤ 5 %
Suitability for microscopy: passes test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

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Danger

Hazard Classifications

Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Skin Corr. 1B - STOT SE 3

target_organs

Respiratory system

Storage Class

8A - Combustible, corrosive hazardous materials

wgk

WGK 3

Regulatory Information

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