14-557-M
B-Raf (V599E) Protein, active, 10 µg
Active, N-terminal GST-tagged recombinant human B-Raf residues 416-end, containing a V599E mutation, for use in Kinase Assays.
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.41
eCl@ss:
32160405
Biological source:
human
Recombinant:
expressed in baculovirus infected Sf21 cells
Mol wt:
Mw 67.3 kDa
Product Name
B-Raf (V599E) Protein, active, 10 µg, Active, N-terminal GST-tagged recombinant human B-Raf residues 416-end, containing a V599E mutation, for use in Kinase Assays.
biological source
human
recombinant
expressed in baculovirus infected Sf21 cells
mol wt
Mw 67.3 kDa
manufacturer/tradename
Upstate®
technique(s)
activity assay: suitable (kinase)
NCBI accession no.
UniProt accession no.
Quality Level
Analysis Note
routinely evaluated by phosphorylation of MBP substrate in vitro
Application
Research Category
Inflammation & Immunology
Inflammation & Immunology
Biochem/physiol Actions
Protein Target: B-Raf
Target Sub-Family: TKL
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
N-terminal GST-tagged recombinant human B-Raf residues 416-end, containing a V599E mutation (also designated V600E)
Product Source: Expressed by baculovirus in Sf21 insect cells
Other Notes
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Packaging
Also available in 250 µg size --call for pricing and availability and reference catalog number 14-557M when ordering the 250 µg size.
Physical form
Glutathione agarose affinity chromatography
Preparation Note
6 months at -70°C
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
Storage Class
10 - Combustible liquids
wgk
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Vanessa C Gray-Schopfer et al.
Cancer metastasis reviews, 24(1), 165-183 (2005-03-24)
Melanoma is a form of skin cancer that has a poor prognosis and which is on the rise in Western populations. If detected early, it is easily treated by surgical excision. However, once melanoma metastasises it is notoriously resistant to
Mathew J Garnett et al.
Cancer cell, 6(4), 313-319 (2004-10-19)
RAF proteins are components of a conserved signaling pathway that regulates cellular responses to extracellular signals. Despite over 20 years of research into the regulation and function of the RAF proteins, it was only realized recently that the B-RAF isoform
Stevan R Hubbard
Cell, 116(6), 764-766 (2004-03-24)
A study by Wan et al. in this issue of Cell demonstrates that the majority of oncogenic mutations in the B-Raf protein kinase result in increased catalytic activity, through disruption of the autoinhibited state of the kinase domain. Surprisingly, several
Kathryn E Mercer et al.
Biochimica et biophysica acta, 1653(1), 25-40 (2003-06-05)
A recent report has shown that activating mutations in the BRAF gene are present in a large percentage of human malignant melanomas and in a proportion of colon cancers. The vast majority of these mutations represent a single nucleotide change
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