CpGenome Direct Prep-96 Bisulfite Modification Kit (192 Reactions)

Methylation of cytosines located 5′ to guanosine is known to have a profound effect on the expression of many eukaryotic genes. In normal cells methylation occurs predominantly in CG-poor regions, while CG-rich areas, called CpG-islands remain unmethylated. The exceptions are the extensive methylation of CpG islands associated with transcriptional inactivation of regulatory regions of imprinted genes and genes on the inactive X-chromosome of females. Aberrant methylation of normally unmethylated CpG islands has been documented as a relatively frequent event in immortalized and transformed cells and has been associated with transcriptional inactivation of defined tumor suppressor genes in human cancers. Hundreds of CpG islands are now known to exhibit the characteristic of hypermethylation in tumors resulting in identification of candidate genes that can be interrogated to determine extent of tumor specific transformation.

For MSP primer design, please use the MethPrime software package. Click here

2X Extraction Buffer Modification Reagent Conversion Buffer Resuspension Buffer Equilibration Buffer Binding Buffer Wash Buffer I Wash Buffer II Elution Buffer Proteinase K Proteinase K Storage Buffer Conversion Plates/Cover films Desalting Plates Collection Plates Elution Plates

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