Brain Derived Neurotrophic Factor, Human, Recombinant, E. coli

Recombinant, human brain drived neurotrophic factor expressed in E. coli. Promotes survival and growth of neurons in the central nervous system. Sequence analysis indicates that brain derived neurotrophic factor is structurally and functionally related to nerve growth factor. mRNA for brain derived neurotrophic factor is found predominantly in the central nervous system, whereas mRNA for nerve growth factor is also found in many non-CNS tissues.

Supports survival of rat basal forebrain primary septal cultured neurons. Induces choline acetyltransferase (EC₅₀ = 50 ng/ml)

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. Avoid freeze/thaw cycles of solutions.

Reconstitute to a concentration of ≥10 µg/ml with sterile distilled H₂O or aqueous buffers. For more dilute solutions, add a stabilizing protein such as 0.1% BSA or HSA.

Erickson, J.T., et al. 1996. J. Neurosci. 16, 5361.
Leibrock, J., et al. 1989. Nature341, 149.

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Toxicity: Standard Handling (A)