Anti-β-Catenin, Dephospho Mouse mAb (8E4)

Mouse monoclonal antibody purified by thiophilic adsorption and size exclusion chromatography. Recognizes the ~90 kDa nuclear dephospho β-catenin protein. Supplied with a control lysate derived from 2x10⁶ LiCl-treated SW480 cells.

Recognizes the ~90 kDa nuclear dephospho-β-catenin in vanadate treated A431 cells. Supplied with a control lysate derived from 2x10⁶ LiCl-treated SW480 cells.

This Anti-β-Catenin, Dephospho Mouse mAb (8E4) is validated for use in ELISA, Immunoblotting, Immunoprecipitation for the detection of β-Catenin, Dephospho.

Mouse

a synthetic peptide corresponding to amino acids 27-37 of mouse β-catenin, conjugated to KLH

ELISA (0.05 µg/ml)

Immunoblotting (1 µg/ml colorimetric, 0.1 µg/ml, chemiluminescence)

Immunoprecipitation (1-10 µg/10⁶ LiCl-treated SW480 cells)

100 µg antibody lyophilized from 2X PBS, sucrose, PEG and 200 µl lyophilized control lysate.

Reconstitute antibody with 1 ml dH₂O. Following reconstitution, refrigerate (4°C) for short-term storage or aliqout, flash-freeze in liquid nitrogen, and deep freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Reconstitute positive control lysate with 200 µl H₂O. After complete solubilization of the proteins add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min. Following reconstitution of positive control, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.

Positive Control
LiCl-treated SW480 cells (included)

SW480 LiCl cell lysate is recommended for immunoblot applications at a concentration of 20 µl/lane (mini gel) for chemiluminescent detection. Variables associated with assay conditions will dictate the proper working dilution.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Standard Handling (A)