Macrophage Colony-Stimulating Factor, Human, Recombinant, E. coli

Recombinant, human macrophage colony-stimulating factor expressed in E. coli. Stimulates formation of macrophage colonies from bone marrow hematopoietic progenitor cells. Induces cell death in HIV-1 infected monocytes. Human M-CSF is active in murine systems and the recombinant cytokine is indistinguishable from the natural M-CSF in all biological assays tested.

ED₅₀ = 0.5-1.5 ng/ml as measured in a cell proliferation assay using the M-CSF-dependent mouse monocytic cell line, M-NFS-60

Lyophilized from sterile-filtered PBS, 50 µg of BSA/µg M-CSF.

Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for 1 month at 4°C or for 3 months at -70°C.

Reconstitute to a concentration of ≥1 mg/ml with sterile PBS containing ≥0.1% HSA or BSA.

Edwards, M., et al. 1998. Bone 22, 325.
Bergamini, A., et al. 1994. Immunol. Lett. 42, 35.
Hattersley, G., et al. 1991. Biochem. Biophys. Res. Commun.177, 526.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Standard Handling (A)