Key Documents

View All Documentation

264150

Caspase-3 Substrate IV, Fluorogenic

Name: Caspase-3 Substrate IV, Fluorogenic
Brand: MM

Fluorogenic caspase-3 substrate.

Synonym(s):

Caspase-3 Substrate IV, Fluorogenic, Z-DEVD-AFC

Select a Size

Change View

About This Item

Empirical Formula (Hill Notation):

C₃₆H₃₈F₃N₅O₁₄

CAS Number:

1177935-21-5

Molecular Weight:

821.71

MDL number:

MFCD01074977

UNSPSC Code:

12352202

NACRES:

NA.77

Assay:

≥95% (HPLC)

Form:

solid

Quality level:

100

Storage condition:

OK to freeze

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

Quality Level

100

assay

≥95% (HPLC)

form

solid

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze

color

white to off-white

solubility

DMSO: 10 mg/mL

fluorescence

λ_ex ~400 nm, λ_em ~505 nm

shipped in

ambient

storage temp.

−20°C

SMILES string

FC(F)(F)C1=CC(=O)Oc2c1ccc(c2)NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)OCc3ccccc3)CC(=O)OC)CCC(=O)OC)C(C)C)CC(=O)OC

InChI key

NLDGPABVDARZCY-KIAQLKRMSA-N

Description

General description

Fluorogenic CPP-32/apopain substrate. Also acts as a substrate for granzyme B-processed ICE-LAP3. Reaction can be monitored by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore. Excitation max: ~400 nm; emission max: ~505 nm.

Fluorogenic caspase-3 substrate. Also acts as a substrate for granzyme B-processed ICE-LAP3. Reaction can be monitored by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore. Also a substrate for caspase-6, caspase-7, caspase-8, and caspase-10.

Biochem/physiol Actions

Cell permeable: no

Primary Target
Fluorogenic caspase-3 substrate.

Product does not compete with ATP.

Reversible: no

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for 3 months at -20°C.

Other Notes

Keramaris, E., et al. 2000. Mol. Cell Neurosci. 15, 368.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Kluck, R.M., et al. 1997. Science275, 1132.
Talanian, J., et al. 1997. J. Biol. Chem.272, 9677.
Chinnaiyan, A.M., et al. 1996. Curr. Biol.6, 897.
Nicholson, D.W., et al. 1995. Nature376, 37.

Z-Asp-Glu-Val-Asp-AFC

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c