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5.08940

Millipore

G 418 Sulfate, Sterile-Filtered Solution in 100 mM HEPES, Cell Culture Tested

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Synonym(s):
G 418 Sulfate, Sterile-Filtered Solution in 100 mM HEPES, Cell Culture Tested, G418, G 418
Empirical Formula (Hill Notation):
C20H40N4O10 · 2H2SO4
CAS Number:
108321-42-2
Molecular Weight:
692.71
MDL number:
MFCD00058314

Assay

≥98% (TLC)

Quality Level

200

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles
protect from light

storage temp.

−20°C

InChI

1S/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19;2*1-5(2,3)4/h6-19,24-30H,4-5,21-23H2,1-3H3;2*(H2,1,2,3,4)/t6?,7-,8+,9+,10+,11-,12-,13-,14+,15+,16-,17-,18+,19-,20+;;/m0../s1

InChI key

UHEPSJJJMTWUCP-NKCAIAFTSA-N

General description

Recommended reaction conditions: The optimal concentration of G418 for selection of resistance will vary according to the organism and/or cell type under investigation. In general, the concentration of active drug required for selection is as follows: Dictyostelium sp.: 10-100 µg/ml Plant cells: 10-100 µg/ml Yeast cells: 0.5-1.0 µg/ml Mammalian cells: 0.1-2.0 µg/ml

Packaging

Packaged under inert gas

Warning

Toxicity: Harmful (C)

Physical form

Sterile-filtered solution of Cat. No. 345810 supplied at 50 mg/ml in 100 mM HEPES, pH 7.3.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 1 year at -20°C.

Other Notes

Ethier, S.P., and Taback, E. 1993. Cancer Lett.74, 189.
Santerre, R.F., et al. 1991. Methods Mol. Biol.7, 245.
Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol.133, 46.
Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol.163, 8.
Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA79, 5166.
Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA79, 7356.
Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun.101, 1031.
Jimenez, A., and Davies, J. 1980. Nature287, 869.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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