516678-M
4EBP1, His•Tag®, Human, Recombinant, S. frugiperda
4EBP1, His•Tag, Human, is a full-length, human recombinant 4EBP1 fused at the N-terminus to a His•Tag sequence and expressed in S. frugiperda insect cells.
Synonym(s):
PHAS-I, Human, Recombinant
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About This Item
UNSPSC Code:
12352203
Form:
liquid
Assay:
≥75% (SDS-PAGE)
Recombinant:
expressed in insect cells (S. frugiperda)
recombinant
expressed in insect cells (S. frugiperda)
assay
≥75% (SDS-PAGE)
form
liquid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
storage temp.
−70°C
General description
Full-length, recombinant, human 4EBP1 fused at the N-terminus to a His•Tag sequence and expressed in S. frugiperda insect cells. 4EBP1 negatively regulates translation and is inhibited by p70S6K phosphorylation. 4EBP1 is useful as a substrate in the study of p70S6K regulation and inhibitor screening.
Physical form
In 300 mM NaCl, 150 mM Imidazole, 50 mM Sodium Phosphate, 0.2 mM DTT, 0.1 mM PMSF, 25% Glycerol, pH 7.0.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Pause, A., et al. 1994. Nature.371,762.
Tsukiyama-Kohara, K., et al. 1996. Genomics.38,353.
Tsukiyama-Kohara, K., et al. 1996. Genomics.38,353.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Regulatory Review (Z)
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 1
Certificates of Analysis (COA)
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