form
liquid
Quality Level
manufacturer/tradename
Novagen®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
storage temp.
−70°C
General description
The pSTBlue-1 vector is a multi-purpose cloning vector featuring a versatile multiple cloning region, blue/white screening, dual opposed T7/SP6 promoters and dual kanamycin/ampicillin resistance. Restriction sites producing 4-base 3′ overhangs are conveniently positioned near each end of the polylinker to facilitate the generation of unidirectional deletions using exonuclease III.
For enhanced convenience, please consider Introductory pSTBlue-1 Perfectly Blunt®Cloning Kit, or pT7Blue™-1 Perfectly Blunt Cloning Kit.
For enhanced convenience, please consider Introductory pSTBlue-1 Perfectly Blunt®Cloning Kit, or pT7Blue™-1 Perfectly Blunt Cloning Kit.
Other Notes
20 rxn40 rxn
•2 × 0.5 µg4 × 0.5 µgBlunt Vector
•10 µl10 µlPositive Control Insert
•100 µl2 × 100 µlEnd Conversion Mix
•2 × 0.5 µg4 × 0.5 µgBlunt Vector
•10 µl10 µlPositive Control Insert
•100 µl2 × 100 µlEnd Conversion Mix
Novy, R.E., et al. 1996. inNovations6, 7. Clark, J.M. 1988. Nucleic Acids Res. 16, 9677. Brownstein, J.M., et al. 1996. BioTechniques20, 1004. Magnuson, V.L., et al. 1996. BioTechniques21, 700. Hu, G. 1993. DNA and Cell Biology12, 763.
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
PT7BLUE is a trademark of Merck KGaA, Darmstadt, Germany
Perfectly Blunt is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class Code
10 - Combustible liquids
Certificates of Analysis (COA)
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