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AB9562

Sigma-Aldrich

Anti-Huntingtin phosphoSer 421 Antibody

Chemicon®, from rabbit

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

western blot: suitable

NCBI accession no.

NM_002111.6

UniProt accession no.

P42858

shipped in

dry ice

target post-translational modification

phosphorylation (pSer421)

Gene Information

human ... HTT(3064), SLC6A4(6532)

Specificity

Recognizes Huntingtin protein phosphorylated at serine 421.Minimal reactivity is expected with the non-phosphorylated form of the protein.

Immunogen

Epitope: phosphoSer 421
Synthetic peptide from human Huntingtin protein. It is expected that the antibody may cross react with rat and mouse due to conservation of the immunogen sequence (8/9).

Application

Detect Huntingtin using this Anti-Huntingtin Antibody, phosphoSer 421 validated for use in WB.
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Western blotting: 1:500-1:3,000 using ECL. Reacts with the ~190 kD Huntingtin protein in unstimulated human PC-3 whole cell lysate. Additional bands at ~130 may also be detected.

ELISA: 1:10,000-1:40,000

Optimal working dilutions must be determined by the end user.

Physical form

Affinity purified immunoglobulin. Liquid in 0.02M Potassium phosphate, 0.15M Sodium Chloride with 0.01% sodium azide.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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