ABE1378
Anti-RAD18 Antibody
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
conjugate
unconjugated
Quality Level
antibody form
purified antibody
clone
polyclonal
purified by
using protein G
species reactivity
rabbit, human
concentration
(Please refer to lot specific datasheet.)
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
UniProt accession no.
target post-translational modification
unmodified
General description
GST-tagged recombinant human RAD18 C-terminal fragment.
~65/75 kDa observed. Ubiquination of RAD18 can be detected (Miyase, S., et al. (2005). J. Biol. Chem. 280:515-524). Uncharacterized band(s) may appear in some lysates.
Application
Epitope: C-terminal region.
Biochem/physiol Actions
This polyclonal antibody detected both ubiquitinated and non-ubiquitinated human RAD18 by targeting the C-terminal region of human Rad18 (Miyase, S., et al. (2005). J. Biol. Chem. 280(1):515-524; Tateishi, S., et al. (2003). Mol. Cell. Biol. 23(2):474-481).
Physical form
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in U2OS cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected RAD18 in 10 µg of U2OS cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected RAD18 in 10 µg of U2OS cell lysate.
Other Notes
Human. Predicted to react with non-human primate based on 100% sequence homology.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Regulatory Information
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