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EZHL

Human Leptin ELISA Kit

measures and quantify Leptin levels in 25 μL serum or plasma

Synonym(s):

Leptin, Obesity factor

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.32
Gene information:
human ... LEP(3952)
Input:
sample type cell culture supernatant
sample type plasma (K2 EDTA)
sample type serum
Assay range:
accuracy: 94-114%, intra-assay cv: 1.4-4.9
(Sensitive Assay), intra-assay cv: 2.6-4.6, linearity: 80-105%, linearity: 96-125%
(sensitive assay), sensitivity: 0.125 ng/mL
(sensitive assay), sensitivity: 0.125-20 ng/mL, sensitivity: 0.5 ng/mL, standard curve range: 0.5-100 ng/mL
Species reactivity:
human
Storage temp.:
2-8°C
Shipped in:
wet ice
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Product Name

Human Leptin "Dual Range" ELISA, This Human Leptin "Dual Range" ELISA is used to measure & quantify Leptin levels in Metabolism & Endocrine & Neuroscience research.

Quality Level

species reactivity

human

packaging

kit of 1 × 96 wells

parameter

25 μL sample volume (4hr assay)

assay range

accuracy: 94-114%, intra-assay cv: 1.4-4.9
(Sensitive Assay), intra-assay cv: 2.6-4.6, linearity: 80-105%, linearity: 96-125%
(sensitive assay), sensitivity: 0.125 ng/mL
(sensitive assay), sensitivity: 0.125-20 ng/mL, sensitivity: 0.5 ng/mL, standard curve range: 0.5-100 ng/mL

technique(s)

ELISA: suitable

input

sample type cell culture supernatant
sample type plasma (K2 EDTA)
sample type serum

NCBI accession no.

UniProt accession no.

application(s)

research use

detection method

colorimetric (450nm/590nm)

shipped in

wet ice

storage temp.

2-8°C

Gene Information

human ... LEP(3952)

General description

Leptin is a hormone that is secreted by the adipose tissue. It is also produced by the gastrointestinal tract and placenta. Leptin consists of a four-helix bundle. Leptin participates in regulating appetite, body weight, and energy expenditure. Excessive leptin is associated with cardiovascular complications like hypertension, coronary heart disease, and stroke. The LEP gene is mapped on the human chromosome at 7q32.1. Human Leptin "Dual Range" enzyme-linked immunosorbent assay (ELISA) kit is used for the non-radioactive quantification of human leptin in serum, plasma, and other biological media.

Application

Human Leptin "Dual Range" ELISA kit has been used in the analysis of leptin in:
  • blood samples
  • serum samples
  • plasma samples



pictograms

CorrosionExclamation mark

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1 - Skin Sens. 1

wgk

WGK 3

Storage Class

10 - Combustible liquids



Certificates of Analysis (COA)

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Related Content


Gender dimorphism in skeletal muscle leptin receptors, serum leptin and insulin sensitivity.
Borja Guerra,Teresa Fuentes,Safira Delgado-Guerra,Amelia Guadalupe-Grau,Hugo Olmedillas et al.
Testing null
A study on biomarkers, cytokines, and growth factors in children with burn injuries.
N M Abdel-Hafez,Y Saleh Hassan,T H El-Metwally
Annals of Burns and Fire Disasters null
P Prolo et al.
The international journal of biochemistry & cell biology, 30(12), 1285-1290 (1999-01-30)
Leptin is an adipocyte hormone that signals nutritional status to the central nervous system (CNS) and peripheral organs. Leptin is also synthetized in the placenta and in gastrointestinal tract, although its role in these tissues is not yet clear. Circulating



Global Trade Item Number

SKUGTIN
EZHL-80SK08436037122705
EZHL-80BK04053252436963