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Merck
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HSTCMAG28SPMX21

MILLIPLEX® Human High Sensitivity T Cell Panel

Premixed 96-well Human High Sensitivity T Cell 21-Plex Panel

Synonym(s):

Human high-sensitivity cytokine multiplex kit, Luminex® human high-sensitivity cytokine immunoassay, Millipore human high-sensitivity cytokine panel

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.47
eCl@ss:
32161000

Key Documents

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Product Name

MILLIPLEX® Human High Sensitivity T Cell Panel, Premixed 96-well Human High Sensitivity T Cell 21-Plex Panel

species reactivity

human

Quality Level

200

packaging

pkg of 1 ea

manufacturer/tradename

Milliplex®

assay range

accuracy: 98-107%
sensitivity: 0.11-8.17 pg/mL
(Overnight Incubation minDC)
standard curve range: 0.18-750 pg/mL
(IL-6)
standard curve range: 0.24-1,000 pg/mL
(IL-13)
standard curve range: 0.24-1,000 pg/mL
(IL-21)
standard curve range: 0.31-1,250 pg/mL
(IL-8)
standard curve range: 0.31-1,250 pg/mL
(MIP-1α)
standard curve range: 0.37-1,500 pg/mL
(IL-7)
standard curve range: 0.45-1,750 pg/mL
(TNFα)
standard curve range: 0.49-2,000 pg/mL
(IL-1β)
standard curve range: 0.49-2,000 pg/mL
(IL-12 (p70))
standard curve range: 0.49-2,000 pg/mL
(IL-2)
standard curve range: 0.49-2,000 pg/mL
(IL-5)
standard curve range: 0.61-2,500 pg/mL
(IFNγ)
standard curve range: 0.61-2,500 pg/mL
(MIP-3α)
standard curve range: 0.73-3,000 pg/mL
(IL-17A)
standard curve range: 0.92-3,750 pg/mL
(MIP-1β)
standard curve range: 1.22-5,000 pg/mL
(GM-CSF)
standard curve range: 1.46-6,000 pg/mL
(IL-10)
standard curve range: 1.46-6,000 pg/mL
(ITAC)
standard curve range: 1.83-7,500 pg/mL
(IL-4)
standard curve range: 18.3-75,000 pg/mL
(Fractalkine)
standard curve range: 7.93-32,500 pg/mL
(IL-23)
inter-assay cv: <15%
intra-assay cv: <10%

technique(s)

multiplexing: suitable

input

serum
cell culture supernatant

detection method

fluorometric (Luminex® xMAP® technology)

shipped in

wet ice

storage temp.

2-8°C

Related Categories

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Application

MILLIPLEX® Qualified assays undergo rigourous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze 21 analytes in human serum, plasma, and cell culture supernatants.Analytes included: Fractalkine/CX3CL1, GM-CSF, IFN-gamma, IL-1beta, IL-2, IL-4, IL-5, IL-6, IL-7, IL8/CXCL8, IL-10, IL-12 (p70), IL-13, IL17A/CTLA8, IL-21, IL-23, ITAC/EXCL11, MIP-1α/CCL3, MIP-1β/CCL4, MIP-3α/CCL20 and TNFα.Assay Characteristics: Refer to kit protocol for assay cross-reactivity, sensitivity, precision, and accuracy.

Disclaimer

For research use only. Not for use in diagnostic procedures.Label License/Sticker for Assay Product:By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.

Features and Benefits

Multiplex Capability: Simultaneous quantification of up to 21 cytokines and chemokines in a single sample using Luminex® xMAP® technology.High Sensitivity: Detects cytokines at picogram levels (as low as 0.11 pg/mL), ideal for low-abundance targets in serum, plasma, and cell culture supernatants.Comprehensive Coverage: Includes key T cell-related cytokines such as IL-2, IL-4, IL-10, IL-17A, IFNγ, TNFα, and more.Neuroimmune Axis Coverage: Includes IL-6 and MIP-1β, implicated in neuroinflammatory disorders, allowing you to decipher cytokine roles in Alzheimer′s, depression, or stroke recovery.Metabolic Inflammation Markers: Quantify IL-1β and IL-13, linked to insulin resistance and adipose inflammation to study obesity-driven metabolic syndrome or type 2 diabetes mechanisms.

General description

Cytokines are immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses. One of the regulatory mechanisms of the immune system, cytokines act at the recognition, activation, or effector phases of an immune response, modulating the development and functional activities of the subtypes of T cells, B cells and myeloid cells. Consequently, research involving cytokines plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, especially those responses that make up the inflammatory process. Low levels of inflammation are involved in many sub-clinical disease states, such as autoimmune disease, cardiovascular disease, diabetes, neurological disorders, and cancer. Measuring picogram levels of cytokines, therefore, is critical for understanding the pathogenesis of these diseases.

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Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

target_organs

Respiratory Tract

Storage Class

10 - Combustible liquids

Regulatory Information

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Chest, 154(6), 1301-1310 (2018-07-22)
Previously healthy firefighters with World Trade Center (WTC) dust exposure developed airway disease. Risk factors for irritant-associated asthma/COPD overlap are poorly defined. This study included 2,137 WTC-exposed firefighters who underwent a clinically indicated bronchodilator pulmonary function test (BD-PFT) between 9/11/2001
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Journal of neuroinflammation, 15(1), 63-63 (2018-03-02)
Low-grade inflammation has been repeatedly associated with both excess weight and psychosis. However, no previous studies have addressed the direct effect of body mass index (BMI) on basal serum cytokines in individuals with first-episode psychosis (FEP). The aim of this
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Off-tumor targeting of human antigens is difficult to predict in preclinical animal studies and can lead to serious adverse effects in patients. To address this, we developed a mouse model with stable and tunable human Her2 (hHer2) expression on normal
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