Anti-GAPDH Antibody, clone 6C5, Alexa Fluor^™ 647 conjugate

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an ubiquitous glycolytic enzyme present in reasonably high levels in almost all tissues. The complete and functional enzyme is a tetramer with each of four identical subunits occupying the vertex of a tetrahedron. Its binding domains also include one principally interacting with NAD+ and another interacting with glyceraldehyde 3-phosphate (GAP). As a ′house-keeping′ enzyme, it catalyzes the synthesis of 1,3-biphosphoglycerate, a "high energy" intermediate used for the synthesis of ATP. Besides its cytoplasmic role in metabolism, it is also involved in the initial stages of apoptosis or oxidative stress response where it is translocated to the nucleus. Such actions may reflect the role of GAPDH in DNA repair or as one nuclear carrier for apoptotic molecules. GAPDH can also bind specifically to proteins implicated in the pathogenesis of a variety of neurodegenerative disorders, including the beta-amyloid precursor protein and the Huntingtin protein where reduced function of GAPDH is associated with Alzheimer′s and Huntington′s disease fibroblasts. GAPDH has also been identified as a potential target for nitric oxide (NO)-mediated cellular toxicity.

~38 kDa observed

Glyceraldehyde-3-phosphate dehydrogenase from rabbit muscle.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected GAPDH in NIH/3T3 cells.
The unconjugated version of this antibody (Cat. No. MAB374) has been demonstrated to perform in WB, ELISA, ICC, IHC, and IP.

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

This Anti-GAPDH Antibody, clone 6C5, Alexa Fluor^™ 647 conjugate is validated for use in Immunocytochemistry for the detection of GAPDH.

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from skeletal muscle. Antibody also recognizes cardiac GAPDH. GAPDH enzyme is detected in many non-muscle cells lines including HeLa, HCT-116 cells, U937 and THP-1 cells among others.

It has been reported that this antibody does not react with GAPDH from E. coli.

Protein A purified

Purified mouse conjugate antibody in buffer containing PBS 15 mg/ml BSA and 0.1% Sodium Azide..

Stable for 1 year at room temperature from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Evaluated by Immunocytochemistry in HUVEC cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected GAPDH in HUVEC cells.

Concentration: Please refer to lot specific datasheet.

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