Anti-Pax6 Antibody, clone AD2.38

Paired box protein Pax-6 (UniProt: P26367; also known as Aniridia type II protein, Oculorhombin, Pax-6) is encoded by the PAX6 (also known as AN2) gene (Gene ID: 5080) in human. Pax6 is a transcription factor that has important functions in the development of the eye, nose, central nervous system and pancreas. It is shown to be essential for the differentiation of pancreatic islet alpha cells. It has two highly conserved DNA binding domains, a paired domain and a homeodomain that is followed by a proline, serine, and threonine-rich trans-activation domain. Its C-terminal peptide region is shown to modulate DNA binding by the homeodomain. It is expressed in the developing eye and brain and in retina it peaks at fetal days 51-60. Pax6 competes with PAX4 in binding to a common element in the glucagon, insulin, and somatostatin promoters. Pax6 is reported to regulate specification of the ventral neuron subtypes by establishing the correct progenitor domains. Mutations in PAX6 gene are known to cause Aniridia, a disorder characterized by complete absence of the iris or extreme iris hypoplasia.

Recombinant human Pax6.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected Pax6 in human cerebellum tissue sections

Immunocytochemistry Analysis: A representative lot detected Pax6 in Immunocytochemistry applications

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Clone AD2.38 is a mouse monoclonal antibody that detects Pax-6 protein.

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stable for 1 year at +2°C to +8°C from date of receipt.

Evaluated by Western Blotting in E13-14 Mouse brain tissue lysate.

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

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