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MABF1995

Sigma-Aldrich

Anti-PvRBP2b, clone 3E9

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
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conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

monoclonal

mol wt

(~120-150 kDa observed; 331.44 kDa calculated (for full length 2pVRBP2b). Uncharacterized bands may be observed in some lysate(s).)

purified by

using protein G

species reactivity

mouse, (parasite)

isotype

IgG2aκ

Protein ID accession no.

UniProt accession no.

target post-translational modification

unmodified

Application

Anti-PvRBP2b, clone 3E9, Cat. No. MABF1995, is a mouse monoclonal antibody that detects Reticulocyte-binding protein 2 in Plasmodium vivax and has been tested for use in ELISA, Flow Cytometry, Inhibition/ Function Assay, and Western Blotting.
ELISA Flow Cytometry Inhibits Activity/Function Western Blotting
Inhibits Activity/Function Analysis: A representative lot inhibited PvRBP2b binding to reticulocytes and blocked the invasion of P. vivax into reticulocytes. (Gruszczyk, J., et. al. (2018). Science. 359(6371):48-55).

Flow Cytometry Analysis: A representative lot detected PvRBP2b in Flow Cytometry applications (Gruszczyk, J., et. al. (2018). Science. 359(6371):48-55).

Enzyme Immunoassay Analysis: A representative lot detected PvRBP2b in ELISA applications (Gruszczyk, J., et. al. (2018). Science. 359(6371):48-55).

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Biochem/physiol Actions

Clone 3E9 is a mouse monoclonal antibody that specifically detects Reticulocyte binding protein 2b (PvRBP2b) in Plasmodium vivax.

Features and Benefits

His-tagged recombinant fragment corresponding to 1294 amino acids from the N-terminal half of Plasmodium vivax Reticulocyte-binding protein 2 (PvRBP2b).

Preparation Note

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Purified mouse monoclonal antibody IgG2a in PBS without azide.

Other Notes

Evaluated by Western Blotting in Plasmodium vivax under non-reducing conditions.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected purified Reticulocyte-binding protein from Plasmodium vivax (2PvRBP2b) under non-reducing conditions.
Reticulocyte-binding protein 2 (UniProt: Q00799; also known as PvRBP2) is encoded by the RBP-2 (also known as RBP2) gene in Plasmodium species. PvRBP2b is an alpha-helical protein, comprising 10 alpha-helices and two very short antiparallel beta-sheets, each comprising two beta-strands. It plays a critical role in reticulocyte recognition and invasion by Plasmodium vivax. It is synthesized with a signal peptide (aa 1-21), a large extracellular domain (aa 22-2805), a short transmembrane domain (aa 2806-2826), and a cytoplasmic tail (aa 2827-2867). Plasmodium vivax is shown to preferentially invade reticulocytes that express high levels of transferrin receptor 1 (TfR1). PvRBP2b is expressed during the late-stage of Plasmodium vivax and its binding has been shown to directly correlate with the levels of TfR1 on reticulocyte surface. Treatment of reticulocytes with trypsin or chymotrypsin can cleave TfR1 from reticulocyte surface and prevent PvRBP2b binding. Clone 3E9 is shown to inhibit reticulocyte binding and block the entry of Plasmodium vivax into reticulocytes. This antibody binds to the side of the N-terminal domain of PvRBP2b to a region localized between helices alpha 4, 5 and 6. (Ref.: Gruszczyk, J., et al. (2018). Science 359(6371); 48-55; Gruszczyk, J., et al. (2018). Nature. 559(7712); 135-139).
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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