Anti-SARS-CoV ORF7a Antibody, clone 3C9

ORF7a protein (UniProt: P59635; also known as Accessory protein 7a, Protein U122, Protein X4) is encoded by the 7a gene (Gene ID: 1489674) in Severe acute respiratory syndrome coronavirus (SARS-CoV). ORF7a protein is expressed by the severe acute respiratory syndrome coronavirus (SARS-CoV) and is not found in other known coronaviruses. It is synthesized with a signal peptide (aa 1-15), which is subsequently cleaved off. Its presence is detected in the mitochondria and the endoplasmic reticulum-Golgi intermediate compartments. The C-terminal region of ORF7a contains a transmembrane domain (aa 97-117) and a short cytoplasmic tail (118 to 122 aa). The transmembrane domain is shown to be important for the insertion of ORF7a into intracellular membranes. The cytoplasmic tail has an endoplasmic reticulum retrieval motif that mediates the transport of ORF7a between the Golgi and the endoplasmic reticulum. ORF7 can induce apoptosis in various cell lines, however, overexpression of Bcl-XL can block ORF-7a induced apoptosis. It is not shown to react with any pro-apoptotic proteins of theBcl-2 family. Its transmembrane domain is also important for its interaction with Bcl-XL and for the induction of apoptosis. Two residues (aa 224, 225) within the transmembrane domain of Bcl-XL are essential for its interaction with ORF7a. SARS-CoV-2 ORF7a is considered as an immunomodulating factor for immune cell binding and can trigger inflammatory responses. It can interact with CD14+ monocytes with high efficiency, compared to the SARS-CoV ORF7a. (Ref.: Zhou, Z., et al. (2021). iScience 24(3); 102187; Tan, YX., et al. (2007). J. Virol. 81(12); 6346-6355).

GST-tagged recombinant, full-length ORF7a protein of SARS-CoV (without the signal peptide).

Quality Control Testing

Evaluated by Western Blotting with recombinant SARS-CoV-2 ORF7a protein.

Western Blotting Analysis: 1:100,000 dilution of this antibody detected recombinant SARS-CoV-2 ORF7a protein.

Tested Applications

Western Blotting Analysis: A representative lot detected SARS-CoV ORF7a in Western Blotting applications (Tan, Y-X., et al. (2007). J Virol. 81(12):6346-55).

Immunofluorescence Analysis: A representative lot detected SARS-CoV ORF7a in Immunofluorescence applications (Tan, Y-X., et al. (2007). J Virol. 81(12):6346-55).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-SARS-CoV ORF7a, clone 3C9, Cat. No. MABF2213, is a mouse monoclonal antibody that detects of SARS-CoV ORF7a protein and is tested for use in Immunofluorescence and Western Blotting.

Clone 3C9 is a mouse monoclonal antibody that detects ORF7a protein of SARS-CoV.

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.