West Nile Virus envelope protein.

~50 kDa observed; 31.88 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Clone E16 is a mouse monoclonal antibody that specifically detects envelope protein of West Nile Virus. It targets an epitope within the DIII lateral ridge region.

Immunocytochemistry Analysis: A representative lot detected West Nile Virus in Immunocytochemistry applications (Thompson, B.S., et. al. (2009). PLoS Pathog. 5(5):e1000453).

Neutralizing Analysis: A representative lot completely inhibited West Nile virus infection at 10 ng/mL. (Oliphant, T., et. al. (2006). J Virol. 80(24):12149-59; Thompson, B.S., et. al. (2009). PLoS Pathog. 5(5):e1000453).

Purified mouse monoclonal antibody IgG2b in PBS without azide.

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Evaluated by Western Blotting with Full-length ectodomain of West Nile Virus envelope protein.

Western Blotting Analysis: 1 µg/mL of this antibody detected Full-length ectodomain of West Nile Virus envelope protein.

Virus.

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