Anti-Myeloid lineage Antibody, clone OX-82

Hematopoietic stem cells (HSC) are known to differentiate either into lymphoid cells or and myeloid cells that include granulocytes, monocytes, megakaryocytes, and dendritic cells. During hematopoiesis, lymphoid lineage cells originate from the lymph tissue and myeloid lineage cells originate from the bone marrow. Hematopoiesis is shown to change over time, with a bias of the dominating HSC towards myeloid development with advancing age. Defects in myelopoiesis is reported to result in several hematologic disorders. Clone OX-82 identifies myeloid cells and stromal elements in a variety of tissues in the adult. It reacts with a 35 kDa molecule expressed on myeloid cells and is useful in isolating pluripotent stem cells from rat bone marrow. (Ref.: Weiskopf, K., et al. (2016). Microbiol Spectr. 4(5); doi:10.1128; Crook, K., and Hunt, SV. (1996). Dev. Immunol. 4(4); 235-246).

Anemic rat bone marrow cells.

Quality Control Testing

Evaluated by Flow Cytometry in Rat neutrophils.

Flow Cytometry Analysis: 1.0 µg of this antibody detected Myeloid lineage in one million Rat neutrophils.

Tested Applications

Fluorescence Activated Cell Sorting (FACS): A representative lot detected Myeloid lineage in Fluorescence Activated Cell Sorting applications (Crook, K., et al. (1996). Dev Immunol. 4(4):235-46).

Flow Cytometry Analysis: A representative lot detected Myeloid lineage in Flow Cytometry applications (Crook, K., et al. (1996). Dev Immunol. 4(4):235-46; Shaaban, S., et al. (2016). Int J Cancer Res. 12(2):69-81).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone OX-82 is a mouse monoclonal antibody that detects Myeloid lineage cells.

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl and 0.05% sodium azide.

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommended storage: +2°C to +8°C.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.