Anti-PRRT1, clone L102/45

Anti-PRRT1, clone L102/45, Cat. No. MABN1867, is a highly specific mouse monoclonal antibody that targets Proline-rich transmembrane protein 1 and has been tested for use in Western Blotting.

Western Blotting

Clone L102/45 is a mouse monoclonal antibody that detects Proline-rich transmembrane protein 1 (PRRT1). It targets an epitope within 66 amino acids from the N-terminal, extracellular domain.

GST-tagged recombinant fragment corresponding to the first 66 amino acids from the N-terminal region of rat Proline-rich transmembrane protein 1 (PRRT1).

Stable for 1 year at 2-8°C from date of receipt.

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Evaluated by Western Blotting in human hippocampus tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected PRRT1 in human hippocampus tissue lysate.

Proline-rich transmembrane protein 1 (UniProt: Q6MG82; also known as Dispanin subfamily D member 1, DSPD1, Synapse differentiation-induced protein 4) is encoded by the Prrt1 (also known as Ng5) gene (gene ID: 406167) in rat. DSPD1 is a highly conserved, multi-pass membrane protein of the CD225/Dispanin family that is expressed in the brain and is detected in excitatory synapses. It contains two transmembrane helices, with both the N and C-termini oriented towards the outside of the cell. It plays an important role during synapse development in regulating AMPA receptor (AMPAR) and PSD-95 content at excitatory synapses. It is a component of the outer core of AMPAR complex. AMPAR complex consists of an inner core that is made of four pore-forming GluA/GRIA proteins and four major auxiliary subunits arranged in two-fold symmetry. The inner core of AMPAR complex is complemented by outer core constituents binding directly to the GluA/GRIA proteins at sites distinct from the interaction sites of the inner core constituents. The proteins of the inner and outer core serve as a platform for other, more peripherally associated AMPAR constituents.

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