Anti-CFDP1 Antibody, clone 15E5.1

Craniofacial development protein 1 (UniProt: Q9UEE9; also known as Bucentaur) is encoded by the CFDP1 (also known as BCNT, CENP-29) gene (Gene ID: 10428) in human. Craniofacial development protein 1 is an evolutionarily conserved member of the Bucentaur (BCNT) family of proteins and displays ubiquitous expression. It contains a BCNT domain of about 80 amino acids in the C-terminal region. Two isoforms of craniofacial development protein 1 are reported that are generated by alternative splicing. It is reported to play a role in embryogenesis and is considered as essential for chromatin/chromosome organization and function. A unique feature of this protein is that it lacks cysteine residues and has an acidic N-terminal region and a lysine/glutamic acid/proline-rich region of 40 amino acids. It also has seven serine phosphorylation sites and Ser250 in the BCNT region is shown to be heavily phosphorylated. (Ref.: Messina, G., et al. (2015). Chromosoma 124(2):153 162; Iwashita, S et al. (2015). Biosci Rep. 35(4): e00228).

~45 kDa observed; 33.59 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

GST-taged recombinant fragment corresponding to the first 111 amino acids from the N-terminal region.

Anti-CFDP1, clone 15E5.1, Cat. No. MABN396, is a highly specific mouse monoclonal antibody that targets CFDP1 and has been tested for use in Western Blotting.

Research Category
Neuroscience

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CFDP1 in 10 µg of Jurkat cell lysate.

Clone 15E5.1 detects Craniofacial development protein 1 in human. It targets an epitope within the first 111 amino acids from the N-terminal half.

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CFDP1 in 10 µg of HEK293 cell lysate.

Concentration: Please refer to lot specific datasheet.

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