Anti-PERK Antibody, clone 4F6.1

Eukaryotic translation initiation factor 2-alpha kinase 3 (EC 2.7.11.1; UniProt Q9NZJ5; also known as Eukaryotic translation initiation factor 2 alpha kinase 3, HsPEK, Pancreatic EIF2-alpha kinase, PRKR-like endoplasmic reticulum kinase) is encoded by the EIF2AK3 (also known as PEK, PERK, WRS) gene (Gene ID 9451) in human. The accumulation of unfolded or misfolded proteins in the endoplasmic reticulum (ER) induces the activation of three main unfold protein response (UPR) pathways, the inositol requiring enzyme-1 (IRE1) pathway, the activating transcription factor 6 (ATF6) pathway, and the protein kinase-like ER kinase (PERK) pathway. The PERK pathway involves PERK auto-phosphorylation/activation, resulting in PERK phosphorylation of eIF2α, which in turn leads to increased ATF4 transcription and ATF4-mediated CAAT/enhancer binding protein (C/EBP) homologous protein (CHOP) upregulation.

~125 kDa observed. Uncharacterized band(s) may appear in some lysates.

Epitope: Internal (C-terminal half)

GST-tagged recombinant protein corresponding to the Internal (C-terminal half) of human PERK.

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected PERK in human liver tissue.

This Anti-PERK Antibody, clone 4F6.1 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of PERK.

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected PERK in 10 µg of HEK293 cell lysate.

Concentration: Please refer to lot specific datasheet.

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